bceB
168
primary bacitracin resistance determinant, [wiki|ABC transporter] (permease), sensory component of the Bce regulatory & detoxification system, protects cell wall biosynthetic targets from inhibition by antimicrobial peptides
locus
BSU_30370
Molecular weight
72.01 kDa
pI
9.79
function
protection of cell wall biosynthetic targets from inhibition by antimicrobial peptides
product
[wiki|ABC transporter] for target protection of cell wall synthesis
essential
no
synonyms
bceB, ytsD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
3,109,397 → 3,111,337
Phenotypes of a mutant
85-fold increased sensitivity to bacitracin [Pubmed|26815905]
The protein
Catalyzed reaction/ biological activity
binds bacitracin, KD: 60 nM [Pubmed|25118291]
inhibits [protein|08F24CA21DC54031F6158AC0C772F3B520E5A849|bceS] autophosphorylation activity in the absence of bacitracin [Pubmed|25118291]
releaves intermediates of the lipid II cycle from the inhibitory interaction with antimicrobial peptides such as bacitracin [pubmed|31871088]
Protein family
[wiki|ABC-4 integral membrane protein family] (according to UniProt)
Structure
[PDB|7TCG] ([protein|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]-[protein|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]) [pubmed|35349335]
[PDB|8G3A] ([protein|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]-[protein|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]-[protein|08F24CA21DC54031F6158AC0C772F3B520E5A849|bceS]) [pubmed|37393310]
[AF|O34741]
Effectors of protein activity
the activity of the [protein|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]-[protein|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB] [wiki|ABC transporter] is inhibited by heptaprenyl pyrophosphate which accumulates in a ''[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]'' mutant [Pubmed|24806199]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]-[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]
description
[Pubmed|12890034]
regulation
induced in the presence of bacitracin, plectasin, mersacidin and actagardine, already at very low levels of bacitracin ([protein|search|BceR]) [Pubmed|12890034,26815905]
regulatory mechanism
[protein|C239C155F5452C86BF6C78190BABBBB07A63DF87|bceR]: activation, [Pubmed|12890034], in [regulon|protein:C239C155F5452C86BF6C78190BABBBB07A63DF87|bceR regulon]
Biological materials
Mutant
GP3218 Δ([gene|C239C155F5452C86BF6C78190BABBBB07A63DF87|bceR]-[gene|08F24CA21DC54031F6158AC0C772F3B520E5A849|bceS]-[gene|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]-[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB])::''cat'', available in [wiki|Jörg Stülke]'s lab
MGNA-A168 (ytsD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/168 NBRP B. subtilis, Japan]
BKE30370 (Δ[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAATGTTCATGCTGCAC, downstream forward: _UP4_TGAAACAGAAAAAGCTCCAG
BKK30370 (Δ[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAATGTTCATGCTGCAC, downstream forward: _UP4_TGAAACAGAAAAAGCTCCAG
labs
[wiki|Susanne Gebhard], Bath, UK
References
Reviews
Original Publications
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Time of last update: 2024-12-22 00:40:29
Author of last update: Jstuelk