yjoB
168
putative substrate-specific molecular chaperone, prevents [protein|C1B89BD3DD27CD566018558D3A7845FB451056F0|gapB] and [protein|B0A1AB6E920E6CBEE45115297599AF9A80972E38|katA] from aggregation at elevated temperatures, AAA family, ATPase activity , similar to cell-division protein [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
locus
BSU_12420
Molecular weight
48.61 kDa
pI
4.73
function
unknown
product
putative substrate-specific molecular chaperone
essential
no
synonyms
yjoB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0465 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,314,453 → 1,315,724
The protein
Protein family
[wiki|AAA ATPase family] (according to UniProt)
Structure
[PDB|7W42] [pubmed|36191235]
[PDB|7W43] (N-terminal domain) [pubmed|36191235]
[PDB|7W46] (complex with ADP) [pubmed|36191235]
[PDB|1LV7] (AAA domain of E. coli [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH], correponds to aa 210 ... 400 of YjoB, 32% identity) [pubmed|12176385]
[AF|O34703]
Paralogous protein(s)
[protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
Expression and Regulation
Operons
genes
[gene|E278CCD5CFD618A0D4ACB92E1E06DCC1B4570ED0|yjoB]
description
[Pubmed|1378051]
sigma factors
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|9987136], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
Biological materials
Mutant
GP3785 (Δ[gene|E278CCD5CFD618A0D4ACB92E1E06DCC1B4570ED0|yjoB]::neo) available in [wiki|Jörg Stülke]'s lab
MGNA-A031 (yjoB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/31 NBRP B. subtilis, Japan]
BKE12420 (Δ[gene|E278CCD5CFD618A0D4ACB92E1E06DCC1B4570ED0|yjoB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATACTCCATTTCA, downstream forward: _UP4_TAAAAGAAAGCACGGGTGTT
BKK12420 (Δ[gene|E278CCD5CFD618A0D4ACB92E1E06DCC1B4570ED0|yjoB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATACTCCATTTCA, downstream forward: _UP4_TAAAAGAAAGCACGGGTGTT
Expression vectors
pGP3828: expression of Strep-[gene|E278CCD5CFD618A0D4ACB92E1E06DCC1B4570ED0|yjoB] by [wiki|pGP380] in B. subtilis suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP3829: expression of [gene|E278CCD5CFD618A0D4ACB92E1E06DCC1B4570ED0|yjoB]-Strep by [wiki|pGP382] in B. subtilis suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP3834 (N-terminal Strep-tag, purification from E. coli, in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
pGP3923: expression of [gene|E278CCD5CFD618A0D4ACB92E1E06DCC1B4570ED0|yjoB] by [wiki|pBQ200] in B. subtilis, available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-11-20 15:31:35
Author of last update: Dennis.wicke