hmp
168
flavohemoglobin, involved in resistance to nitric oxide (NO), essential for long-term survival under anaerobic conditions
locus
BSU_13040
Molecular weight
44.57 kDa
pI
5.7
function
resistance to NO
product
flavohemoglobin
essential
no
synonyms
hmp, ykiA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1018 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,372,792 → 1,373,991
The protein
Catalyzed reaction/ biological activity
NADPH + 2 nitric oxide + 2 O2 --> H+ + NADP+ + 2 nitrate (according to UniProt)
NADH + 2 nitric oxide + 2 O2 --> H+ + NAD+ + 2 nitrate (according to UniProt)
Protein family
globin family (single member, according to UniProt)
C-terminal part: flavoprotein pyridine nucleotide cytochrome reductase family (single member, according to UniProt)
[wiki|Domains]
[wiki|FAD-binding FR-type domain] (aa 152-255) (according to UniProt)
[wiki|Cofactors]
FAD (according to UniProt)
Structure
[PDB|1CQX] (from Ralstonia eutropha, 47% identity) [pubmed|8557026]
[AF|P49852]
Expression and Regulation
Operons
genes
[gene|E272A68088B689873CE3269CFDD2DD830F3AB3E4|hmp]
description
[Pubmed|8682784]
regulation
expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]
regulatory mechanism
[protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]: activation, [Pubmed|10972836], in [regulon|protein:1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD regulon]
[protein|EC6697D5D945B7E5083AFED9218748763C443278|nsrR]: repression, [Pubmed|16885456], in [regulon|protein:EC6697D5D945B7E5083AFED9218748763C443278|nsrR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8682784], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|E272A68088B689873CE3269CFDD2DD830F3AB3E4|hmp]-[gene|5688E6CB20A55856F4A4D893CED3D54BA21209AE|ykjA]
description
[Pubmed|22383849]
regulation
expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]
Biological materials
Mutant
BKE13040 (Δ[gene|E272A68088B689873CE3269CFDD2DD830F3AB3E4|hmp]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTATAAACAATCCTTT, downstream forward: _UP4_TGAACACATACATGAAACTC
BKK13040 (Δ[gene|E272A68088B689873CE3269CFDD2DD830F3AB3E4|hmp]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTATAAACAATCCTTT, downstream forward: _UP4_TGAACACATACATGAAACTC
References
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Time of last update: 2025-03-17 00:57:46
Author of last update: Melvin.boenninger