yncD
168
spore coat alanine racemase
locus
BSU_17640
Molecular weight
43.49 kDa
pI
9.59
function
protection of the spore
product
spore coat alanine racemase
essential
no
ec
5.1.1.1
synonyms
yncD, alrB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0787 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,897,941 → 1,899,125
The protein
Catalyzed reaction/ biological activity
L-alanine --> D-alanine (according to UniProt)
Protein family
alanine racemase family (with [protein|CB0B6C69CB5CFF6FE30C3F95991F309C8DE1E344|alr], according to UniProt)
[wiki|Cofactors]
PLP (according to UniProt)
Structure
[PDB|5IRP] (from Bacillus subtilis 100% identity)
[PDB|6Q70] (crystallized with HEPES) [Pubmed|30977502]
[PDB|6Q71] (crystallized with Bis-Tris Propane) [Pubmed|30977502]
[PDB|6Q72] [Pubmed|30977502]
[AF|P94494]
Paralogous protein(s)
[protein|CB0B6C69CB5CFF6FE30C3F95991F309C8DE1E344|alr]
[wiki|Localization]
outer spore coat, localization depends on [protein|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE] [Pubmed|22171814]
Expression and Regulation
Operons
genes
[gene|E267F2B6448E380B354AE091734E4AC4DB5825EF|yncD]
description
[Pubmed|22383849]
regulation
expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|15699190,14523133]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,14523133], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
MGNA-B099 (yncD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1098 NBRP B. subtilis, Japan]
BKE17640 (Δ[gene|E267F2B6448E380B354AE091734E4AC4DB5825EF|yncD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAACGCTTCCTTCCTTC, downstream forward: _UP4_TAGAACATCTTAAAATGCTG
BKK17640 (Δ[gene|E267F2B6448E380B354AE091734E4AC4DB5825EF|yncD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAACGCTTCCTTCCTTC, downstream forward: _UP4_TAGAACATCTTAAAATGCTG
References
Reviews
Original Publications
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Time of last update: 2024-12-21 04:42:23
Author of last update: Jstuelk