yncD

yncD
168

spore coat alanine racemase

locus
BSU_17640
Molecular weight
43.49 kDa
pI
9.59
Protein length
Gene length
function
protection of the spore
product
spore coat alanine racemase
essential
no
ec
5.1.1.1
synonyms
yncD, alrB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0787 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,897,941 → 1,899,125
The protein
Catalyzed reaction/ biological activity
L-alanine --> D-alanine (according to UniProt)
Protein family
alanine racemase family (with [protein|CB0B6C69CB5CFF6FE30C3F95991F309C8DE1E344|alr], according to UniProt)
[wiki|Cofactors]
PLP (according to UniProt)
Structure
[PDB|5IRP] (from Bacillus subtilis 100% identity)
[PDB|6Q70] (crystallized with HEPES) [Pubmed|30977502]
[PDB|6Q71] (crystallized with Bis-Tris Propane) [Pubmed|30977502]
[PDB|6Q72] [Pubmed|30977502]
[AF|P94494]
Paralogous protein(s)
[protein|CB0B6C69CB5CFF6FE30C3F95991F309C8DE1E344|alr]
[wiki|Localization]
outer spore coat, localization depends on [protein|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE] [Pubmed|22171814]
Expression and Regulation
Operons
genes
[gene|E267F2B6448E380B354AE091734E4AC4DB5825EF|yncD]
description
[Pubmed|22383849]
regulation
expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|15699190,14523133]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,14523133], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
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[gene|E267F2B6448E380B354AE091734E4AC4DB5825EF|yncD]

2024-11-20 01:39:02

Jstuelk

51

c92a279fde84555611e560186e24e21ee14bba85

553234D2EBE9519E9FAE4966CFCA17A4F2E1B269

Biological materials
Mutant
MGNA-B099 (yncD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1098 NBRP B. subtilis, Japan]
BKE17640 (Δ[gene|E267F2B6448E380B354AE091734E4AC4DB5825EF|yncD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAACGCTTCCTTCCTTC,  downstream forward: _UP4_TAGAACATCTTAAAATGCTG
BKK17640 (Δ[gene|E267F2B6448E380B354AE091734E4AC4DB5825EF|yncD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAACGCTTCCTTCCTTC,  downstream forward: _UP4_TAGAACATCTTAAAATGCTG
References
Reviews
23202530,35638784
Original Publications
18399999,15699190,14523133,22171814,30977502,33573199

E267F2B6448E380B354AE091734E4AC4DB5825EF

Page visits: 3299

Time of last update: 2024-12-21 04:42:23

Author of last update: Jstuelk