tagV
168
phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid
locus
BSU_35520
Molecular weight
43.05 kDa
pI
9.14
function
transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan
product
phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan
essential
no
synonyms
tagV, yvhJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1316 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,647,406 → 3,648,581
Phenotypes of a mutant
a ''[gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV]'' mutant has no phenoype, the triple ''[gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT] [gene|62A3CC548E033A45F94FE4386EE29711A6A14C6B|tagU] [gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV]'' mutant is unable to grow under normal conditions [Pubmed|21964069]
poor growth of a [gene|A58729F80A0BB6683648E472E385711A6DE4D373|bcrC] [gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV] double mutant [pubmed|39185233]
inactivation suppresses the essentiality of [gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl] [pubmed|39185233]
The protein
Protein family
LytR/CpsA/Psr (LCP) family (with [protein|62A3CC548E033A45F94FE4386EE29711A6A14C6B|tagU] and [protein|68FE85BAA457199D34C9068F38756972CD2D280D|tagT], according to UniProt) [Pubmed|19099556]
[wiki|Cofactors]
Mg2+ [Pubmed|29107701,21964069]
Structure
[PDB|6UF3] (aa 72-332) [pubmed|31969390]
[PDB|3NXH]
[AF|P96499]
Paralogous protein(s)
[protein|62A3CC548E033A45F94FE4386EE29711A6A14C6B|tagU], [protein|68FE85BAA457199D34C9068F38756972CD2D280D|tagT]
[wiki|Localization]
cytoplasmic membrane (with extracytoplasmic catalaytic domain) [Pubmed|21964069]
Expression and Regulation
Operons
genes
[gene|8F46DEAD6195B566E1A5FDC6E6ED9D29969F4AFA|yvyE]-[gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV]
description
[Pubmed|22383849]
regulation
repressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
Biological materials
Mutant
MGNA-A344 (yvhJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/344 NBRP B. subtilis, Japan]
BKE35520 (Δ[gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACGCACTCTAACGCGTTCAG, downstream forward: _UP4_TAAAAAATGCCCGGTCCTTT
BKK35520 (Δ[gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACGCACTCTAACGCGTTCAG, downstream forward: _UP4_TAAAAAATGCCCGGTCCTTT
References
Reviews
Original Publications
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Time of last update: 2024-12-30 04:44:42
Author of last update: Jstuelk