cshA
168
DEAD-box RNA helicase, contributes to efficient 3' exonucleolytic mRNA decay (together with [protein|64C6D783FF3F41C81B216F798A1DC8071345B1ED|PNPase]), important for adaptation to low temperatures
locus
BSU_04580
Molecular weight
57.11 kDa
pI
9.89
function
RNA helicase
product
DEAD-box RNA helicase
essential
no
synonyms
cshA, ydbR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0513 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
511,106 → 512,641
Phenotypes of a mutant
poor growth at low temperatures (16 to 20°C) [Pubmed|23175651]
reduced number of [wiki|ribosome]s [Pubmed|23175651]
no expression of the [gene|C87E74FB67B53DD1C68916AA121388C60CE66E92|frlB]-[gene|9E3FF9D0B33D7EFEA3B61FF863BB46CC6DFD4F9B|frlO]-[gene|8256CAAEC591E40A8CB172FF053E03BFFD5A4B54|frlN]-[gene|1F245030D0C81DC96FC0642199CA00579CF46D06|frlM]-[gene|C3823AFED3E5C3D1EA04C38D861239468C9E6349|frlD] operon [Pubmed|23175651]
strongly increased expression of the [gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]-[gene|F6C3B267106E7510FABECF0B13ABF222901689A9|pftB] operon [Pubmed|23175651]
transcription profile resulting from [gene|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA] deletion: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE36877 GEO] [Pubmed|23175651]
The protein
Catalyzed reaction/ biological activity
RNA helicase
required for [wiki|ribosome] assembly (biogenesis of the large subunit) [Pubmed|23175651]
unwind duplex RNA with or without overhangs [pubmed|28238534]
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
Protein family
[wiki|helicase family] (according to UniProt)
[wiki|DEAD-box RNA helicases] (according to UniProt)
[wiki|Domains]
[wiki|Helicase ATP-binding domain] (aa 34-204) (according to UniProt)
[wiki|Helicase C-terminal domain] (aa 215-375) (according to UniProt)
Structure
[PDB|5IVL] [Pubmed|28238534]
[AF|P96614]
Paralogous protein(s)
[protein|EACD0C84AA3CE41EF05EAA3D25AA1153293EB562|deaD], [protein|0362D937D985846AEE4D0DEA57B62F915E8D35E5|yfmL], [protein|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]
[wiki|Localization]
cytoplasma, colocalizes with the ribosomes [Pubmed|16352840,27708634], may also associate with the cell membrane [Pubmed|20572937]
Expression and Regulation
Operons
genes
[gene|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA]
description
[pubmed|22383849]
Biological materials
Mutant
GP1035 (Δ[gene|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA]::aphA3), available in [wiki|Jörg Stülke]'s lab [pubmed|23175651]
GP1083 (Δ[gene|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA]::cat), available in [wiki|Jörg Stülke]'s lab
BKE04580 (Δ[gene|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTATAAAACTGCCCTTT, downstream forward: _UP4_TAATTTGATCGATTCAGAGC
BKK04580 (Δ[gene|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTATAAAACTGCCCTTT, downstream forward: _UP4_TAATTTGATCGATTCAGAGC
Expression vectors
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP382]: pGP1387, available in [wiki|Jörg Stülke]'s lab
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [wiki|SPINE], expression from the native chromomsomal site: GP1026 (aphA3), available in [wiki|Jörg Stülke]'s lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP1386, available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab, [pubmed|20572937]
FLAG-tag construct
GP1010 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
GP1074 (tet), available in [wiki|Jörg Stülke]'s lab
GFP fusion
pGP1369 for chromosomal expression of CshA-YFP, available in [wiki|Jörg Stülke]'s lab
''B. subtilis'' GP1081 cshA-gfp spc, available in [wiki|Jörg Stülke]'s lab,
GP1721 (in [wiki|pBP43]), expression of '' cshA-GFP''::''spc'' under the native promoter, available in [wiki|Jörg Stülke]'s lab [Pubmed|27708634]
labs
[wiki|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-22 02:37:57
Author of last update: Jstuelk