tsaE
168
protein kinase, required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA
locus
BSU_05910
Molecular weight
17.76 kDa
pI
4.6
function
control of tRNA modification
product
protein kinase involved in tRNA modification
essential
no
synonyms
tsaE, ydiB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0802 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
641,654 → 642,130
Phenotypes of a mutant
reduced growth rate [Pubmed|19246765]
increased sensitivity to paraquat [pubmed|28890133]
The protein
Catalyzed reaction/ biological activity
biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([protein|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]-[protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]) [Pubmed|23072323,21183954]
autophosphorylation on Ser-60, Thr-55, Thr-62, and Thr-64 [pubmed|28890133]
phosphorylation of [protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD] [pubmed|28890133]
Protein family
TsaE family (single member, according to UniProt)
[wiki|Cofactors]
Mg2+ [pubmed|28890133]
Structure
[PDB|5MVR] [Pubmed|28890133]
[AF|O05515]
Modification
autophosphorylation on Ser-60, Thr-55, Thr-62, and Thr-64 [pubmed|28890133]
Kinetic information
K(M) for ATP: 60 myM, V(max) 10 nmol/min [Pubmed|19246765]
[wiki|Localization]
cytoplasm with the exception of the nucleoid region [Pubmed|19246765]
Additional information
ATPase activity is required for ''in vivo'' function of the protein [Pubmed|19246765]
Expression and Regulation
Operons
genes
[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]-[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]-[gene|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|rimI]-[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]
description
[Pubmed|22383849]
Biological materials
Mutant
BKE05910 (Δ[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCTTCACACGAGTCACCC, downstream forward: _UP4_ATGCTTTGTGAGGAGTTAAG
BKK05910 (Δ[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCTTCACACGAGTCACCC, downstream forward: _UP4_ATGCTTTGTGAGGAGTTAAG
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP833, available in [wiki|Stülke] lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP841, available in [wiki|Stülke] lab
References
Page visits: 4994
Time of last update: 2024-12-22 02:30:53
Author of last update: Jstuelk