bslA
168
bacterial hydrophobin, forms water-repellent surface layer of the biofilm, required for sliding, inhibitor of [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA] autophosphorylation, and subsequently of entry into [wiki|sporulation]
locus
BSU_31080
Molecular weight
19.11 kDa
pI
9.99
function
[wiki|biofilm formation], control of entry into [wiki|sporulation] via the [wiki|phosphorelay]
product
biofilm surface layer, inhibitor of [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA] autophosphorylation
essential
no
synonyms
bslA, yuaB, sivB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
3,187,503 → 3,188,048
Phenotypes of a mutant
reduced colony complexity [Pubmed|21742882]
loss of surface repellency of the biofilm [Pubmed|22571672]
diminished [wiki|sliding] of ''B. subtilis'' natto [Pubmed|26152584]
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The protein
Catalyzed reaction/ biological activity
inhibits [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA] autophophorylation [Pubmed|23335417]
contributes to the surface stiffness and increases the surface roughness of NCIB 3610 bioflms [Pubmed|26873313]
Protein family
BslA/BslB family (with [protein|E5E2994D96FCECEE75E15136A08308C7B6C1C183|sivA], according to UniProt)
Structure
[PDB|4BHU] [Pubmed|23904481]
[AF|P71014]
Paralogous protein(s)
[protein|E5E2994D96FCECEE75E15136A08308C7B6C1C183|sivA]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862]
biofilm matrix in an extracellular polysaccharide-dependent manner [Pubmed|22571672]
forms a layer on the biofilm surface [Pubmed|22571672]
Expression and Regulation
Operons
genes
[gene|E06A3392E644FEDC6F46ED41BBE89168F59B76B3|bslA]
description
[Pubmed|22383849]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|18978066], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|lutR]: activation, [Pubmed|24196425], in [regulon|protein:E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|lutR regulon]
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P, [Pubmed|18978066], this might be indirect [Pubmed|21742882], in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
Biological materials
Mutant
MGNA-A218 (yuaB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/218 NBRP B. subtilis, Japan]
''B. subtilis'' W939 ''bslA''::Cm (ATCC6051 based) [Pubmed|17850253]
''B. subtilis'' NRS2097 ''bslA''::Cm (NCIB3610 based) [Pubmed|18978066]
''B. subtilis'' ''bslA''::Cm (168 based) [Pubmed|21097620]
BKE31080 (Δ[gene|E06A3392E644FEDC6F46ED41BBE89168F59B76B3|bslA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAAAATTCCCCCTAA, downstream forward: _UP4_TAATGTAAAAGACCGGTTAA
BKK31080 (Δ[gene|E06A3392E644FEDC6F46ED41BBE89168F59B76B3|bslA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAAAATTCCCCCTAA, downstream forward: _UP4_TAATGTAAAAGACCGGTTAA
Expression vectors
Physpank-bslA based on vector pDR111: pDRyuaB2 [Pubmed|21097620]
lacZ fusion
pNW500 P''bslA-lacZ'' fusion in pDG1663 [Pubmed|18978066]
GFP fusion
P''bslA-gfp'' fusion in pSG1151 vector: pSGyuaB [Pubmed|21097620]
References
Reviews
Original Publications
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Time of last update: 2024-09-09 21:28:47
Author of last update: Jstuelk