bslA

bslA
168

bacterial hydrophobin, forms water-repellent surface layer of the biofilm, required for sliding, inhibitor of KinA autophosphorylation, and subsequently of entry into sporulation

Locus
BSU_31080
Molecular weight
19.11 kDa
Isoelectric point
9.99
Protein length
Gene length
Function
biofilm formation, control of entry into sporulation via the phosphorelay
Product
biofilm surface layer, inhibitor of KinA autophosphorylation
Essential
no
Synonyms
bslA, yuaB, sivB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
3,187,503 → 3,188,048
Phenotypes of a mutant
reduced colony complexity PubMed
loss of surface repellency of the biofilm PubMed
diminished sliding of B. subtilis natto PubMed
The protein
Catalyzed reaction/ biological activity
inhibits KinA autophophorylation PubMed
contributes to the surface stiffness and increases the surface roughness of NCIB 3610 bioflms PubMed
Protein family
BslA/BslB family (with SivA, according to UniProt)
Structure
Paralogous protein(s)
extracellular (signal peptide) PubMed
biofilm matrix in an extracellular polysaccharide-dependent manner PubMed
forms a layer on the biofilm surface PubMed
Expression and Regulation
Operons
Genes
Description
Regulatory mechanism
AbrB: repression, PubMed, in abrB regulon
LutR: activation, PubMed, in lutR regulon
DegU: activation, DegU-P, PubMed, this might be indirect PubMed, in degU regulon
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bslA

2024-12-13 19:32:27

ghost

120

3e63abec5cec8eb1522c6cfb5dea1ad11e365e22

E7DD3197B296DDF0BEBCAD162D11150E39EB6C83

Biological materials
Mutant
MGNA-A218 (yuaB::erm), available at the NBRP B. subtilis, Japan
B. subtilis W939 bslA::Cm (ATCC6051 based) PubMed
B. subtilis NRS2097 bslA::Cm (NCIB3610 based) PubMed
B. subtilis bslA::Cm (168 based) PubMed
BKE31080 (ΔbslA::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATAACAAAATTCCCCCTAA,  downstream forward: _UP4_TAATGTAAAAGACCGGTTAA
BKK31080 (ΔbslA::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATAACAAAATTCCCCCTAA,  downstream forward: _UP4_TAATGTAAAAGACCGGTTAA
Expression vectors
Physpank-bslA based on vector pDR111: pDRyuaB2 PubMed
LacZ fusion
pNW500 PbslA-lacZ fusion in pDG1663 PubMed
GFP fusion
PbslA-gfp fusion in pSG1151 vector: pSGyuaB PubMed
References
Reviews
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Original Publications
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Time of last update: 2025-01-06 19:04:50

Author of last update: Jstuelk