ybcC
168
subunit of a Na+:bicarbonate transporter [protein|CCDDC414E266D86B9027EA67EA565D87357023B3|MpsA]-[protein|E04C67FB0EA3FFEC8FB68440F4661505C3DFDA29|MpsB]
locus
BSU_01845
Molecular weight
99.00 kDa
pI
5.731
function
uptake of bicarbonate
product
subunit of a Na+:bicarbonate transporter
essential
no
ec
null
synonyms
ybcC, mpsB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3002 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
206,941 → 209,556
Phenotypes of a mutant
inactivation of [gene|E04C67FB0EA3FFEC8FB68440F4661505C3DFDA29|ybcC] facilitates the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate [pubmed|33481774]
The protein
Catalyzed reaction/ biological activity
uptake of bicarbonate (with [protein|CCDDC414E266D86B9027EA67EA565D87357023B3|MpsA]) [pubmed|34287032]
Protein family
UPF0753 family (single member, according to UniProt)
Structure
[AF|O34688]
Expression and Regulation
Operons
genes
[gene|CCDDC414E266D86B9027EA67EA565D87357023B3|ndhF]-[gene|E04C67FB0EA3FFEC8FB68440F4661505C3DFDA29|ybcC]-[gene|5D96DF2F32050BBCEC3811843398FE84DE88F6DF|ybcF]-[gene|EBE0360D89E50EEBB84CC853C6E4B4053DBF8925|ybcH]-[gene|6E063903E9E3B770CAACA9DF98DFA540FAE6FD3D|ybcI]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-C509 (ybcC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2507 NBRP B. subtilis, Japan]
BKE01845 (Δ[gene|E04C67FB0EA3FFEC8FB68440F4661505C3DFDA29|ybcC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01845 BGSC] and in [wiki|Jörg Stülke]'s lab as GP3926, [Pubmed|28189581], upstream reverse: _UP1_AATGCCCATCTCAGATTAGC, downstream forward: _UP4_TAATGATGAATCTAAGCATT
BKK01845 (Δ[gene|E04C67FB0EA3FFEC8FB68440F4661505C3DFDA29|ybcC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01845 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATGCCCATCTCAGATTAGC, downstream forward: _UP4_TAATGATGAATCTAAGCATT
Expression vectors
pGP2879: expression of Strep-''ybcC'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
Original Publications
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Time of last update: 2024-11-03 11:30:16
Author of last update: Jstuelk