phoA
168
alkaline phosphatase A
locus
BSU_09410
Molecular weight
50.11 kDa
pI
9.93
function
aquisition of phosphate upon phosphate starvation
product
alkaline phosphatase A
essential
no
ec
3.1.3.1
synonyms
phoA, phoAIV
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1785 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,017,083 → 1,018,468
The protein
Catalyzed reaction/ biological activity
phosphate monoester + H2O --> alcohol + phosphate (according to UniProt)
Protein family
alkaline phosphatase family (with [protein|3B95AB3021280215B6B487A348D78FB5797AEEA6|phoB], according to UniProt)
Structure
[PDB|3A52] (from Shewanella sp., mature protein without signal peptide, 40% identity) [pubmed|20057143]
[AF|P19406]
Paralogous protein(s)
[protein|3B95AB3021280215B6B487A348D78FB5797AEEA6|phoB]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|E0430C16C41440633647976A0F9183E24268E198|phoA]
description
[Pubmed|16291680]
regulation
expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|16291680,8113174]
regulatory mechanism
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|16291680,8113174], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8113174], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|15699190], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
Biological materials
Mutant
1A767 ( ''phoA''::''cat''), [Pubmed|9426145], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A767&Search=1A767 BGSC]
BKE09410 (Δ[gene|E0430C16C41440633647976A0F9183E24268E198|phoA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCTCCTTTAATGTATT, downstream forward: _UP4_TAAAAAGGATGCAGAACGCT
BKK09410 (Δ[gene|E0430C16C41440633647976A0F9183E24268E198|phoA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCTCCTTTAATGTATT, downstream forward: _UP4_TAAAAAGGATGCAGAACGCT
labs
[wiki|Marion Hulett], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]
References
Page visits: 7665
Time of last update: 2025-04-13 02:40:19
Author of last update: Melvin.boenninger