prkD
168
protein kinase D
locus
BSU_02030
Molecular weight
29.91 kDa
pI
6.36
function
unknown
product
protein kinase D
essential
no
ec
2.7.11.1
synonyms
prkD, ybdM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0515 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
223,219 → 223,989
The protein
Catalyzed reaction/ biological activity
ATP + L-seryl-[protein] --> ADP + H+ + O-phospho-L-seryl-[protein] (according to UniProt)
ATP + L-threonyl-[protein] --> ADP + H+ + O-phospho-L-threonyl-[protein] (according to UniProt)
phosphorylates [protein|047CE33C0CC127DAD15D68D63843DF89F93ED3BF|degS] on Ser-76 (in vitro) [Pubmed|21304896]
Protein family
[wiki|protein kinase superfamily] (according to UniProt)
[wiki|Ser/Thr protein kinase family] (according to UniProt)
[wiki|Domains]
[wiki|Protein kinase domain] (aa 25-256) (according to UniProt)
Structure
[PDB|4EQM] (PknB from Staphylococcus aureus, 34% identity in the N-terminal part, aa 18 ... 174) [pubmed|22701750]
[AF|O31435]
Modification
autophosphorylated on Thr-174 (''in vitro'') [Pubmed|20389117]
Biological materials
Mutant
MGNA-B958 (ybdM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1957 NBRP B. subtilis, Japan]
GP578 (aphA3), available in [wiki|Jörg Stülke]'s lab
BKE02030 (Δ[gene|E0097A00C82136BB0B1FB89FBA177E28A4EC3D54|prkD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTGCACTTCCCTTGG, downstream forward: _UP4_CGCGAGGATCTAAACCGGGC
BKK02030 (Δ[gene|E0097A00C82136BB0B1FB89FBA177E28A4EC3D54|prkD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTGCACTTCCCTTGG, downstream forward: _UP4_CGCGAGGATCTAAACCGGGC
Expression vectors
pGP390 (N-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP380]), available in [wiki|Jörg Stülke]'s lab
for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172]: pGP821, available in [wiki|Jörg Stülke]'s lab, [pubmed|20389117]
for expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP1407, available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP830 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
References
Page visits: 5827
Time of last update: 2024-12-19 14:28:02
Author of last update: Melvin.boenninger