tuaD
168
UDP glucose 6-dehydrogenase
locus
BSU_35580
Molecular weight
49.65 kDa
pI
6.11
function
biosynthesis of teichuronic acid
product
UDP glucose 6-dehydrogenase
essential
no
ec
1.1.1.22
synonyms
tuaD, yvhD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1004 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,654,139 → 3,655,524
The protein
Catalyzed reaction/ biological activity
H2O + 2 NAD+ + UDP-α-D-glucose --> 3 H+ + 2 NADH + UDP-α-D-glucuronate (according to UniProt)
Protein family
UDP-glucose/GDP-mannose dehydrogenase family (with [protein|59024C05AA0106719C81E45E3B060A6F710B2690|ytcA] and [protein|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|ugd], according to UniProt)
Structure
[PDB|3GG2] (from Porphyromonas gingivalis, 48% identity)
[AF|O32271]
Modification
phosphorylation on a Tyr residue by [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA] [Pubmed|12970183], dephosphorylated by [protein|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ] [Pubmed|15866923]
Effectors of protein activity
[protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]-dependent phosphorylation stimulates TuaD activity [Pubmed|12970183]
Paralogous protein(s)
[protein|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|ugd], [protein|59024C05AA0106719C81E45E3B060A6F710B2690|ytcA]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|5790E3DB39F31017B557C2D3F428C0348A461DA6|tuaA/2]-[gene|4AC72C556ED02A7DE4E1E8D69A0FC926359ACE28|tuaA/1]-[gene|54F96DE549F78E6C1580949A21C84B27AF26C4C6|tuaB]-[gene|873EE7A8C9BBFC6E96B0957B450BA665E3C9222D|tuaC]-[gene|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|tuaD]-[gene|B89FA71C2924CC0690224AFBFA06DB0FF0D97C0A|tuaE]-[gene|6F0FA23892BB1829174F66C464149DB428A8DE18|tuaF]-[gene|6E4F2BA67FF58085D462F6A6FB6497BDF600FCF6|tuaG]-[gene|737B37DA0DA1642AD5C4A824228BFED55FF0652A|tuaH]
description
[Pubmed|10048024]
regulation
expressed during [wiki|sporulation] in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|16497325,15699190]
regulatory mechanism
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|9611818,10627039], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10048024], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325,15699190], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
Biological materials
Mutant
BKE35580 (Δ[gene|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|tuaD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGCGCTCAACCCTCTCC, downstream forward: _UP4_TAAAAAGAAAAAGTGTTACT
BKK35580 (Δ[gene|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|tuaD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGCGCTCAACCCTCTCC, downstream forward: _UP4_TAAAAAGAAAAAGTGTTACT
References
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Time of last update: 2024-10-14 01:07:20
Author of last update: Melvin.boenninger