sftA
168
DNA translocase, translocation of non-segregated chromosomes prior to septum closure
locus
BSU_29805
Molecular weight
107.00 kDa
pI
5
function
resolution of chromosome dimers
product
DNA translocase
essential
no
synonyms
sftA, ytpS, cdtA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1674 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,049,725 → 3,052,583
The protein
Catalyzed reaction/ biological activity
aids in moving DNA away from the closing septum, binds DNA, DNA-dependent ATPase activity [Pubmed|19818024]
translocation of non-segregated chromosomes prior to septum closure
the two DNA translocases [protein|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA] and [protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE] synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively [Pubmed|21239579]
Protein family
FtsK/SpoIIIE/SftA family (with [protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE], according to UniProt)
[wiki|Domains]
[wiki|FtsK domain] (aa 622-813) (according to UniProt)
Structure
[PDB|2IUT] (FtsK from ''Pseudomonas aeruginosa'', partial, 48% identity, complex with [protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE]) [Pubmed|16916635]
[AF|C0SP86]
Paralogous protein(s)
[protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE]
[wiki|Localization]
peripheral membrane protein (via N-terminal amphipathic helix) [pubmed|35411648]
recruitment to the Z-ring by [protein|DB09F1C36257F511A84A083967A25A9D46744D14|sepF] [pubmed|35411648]
30% of the molecules in the cytoplasm (Homogeneous) [Pubmed|29439991,28110333,16479537]
small fraction at the membrane [Pubmed|28110333]
70% of the molecules are septum-bound, co-localizes with [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] at nascent division sites, recruitment to the septum depends on [protein|search|FtsA ][Pubmed|29439991,19788545]
Expression and Regulation
Operons
genes
[gene|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]-[gene|3BF530AB1B6A3DB3671FC046E2B38CA1936E6F78|murC]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A438 (ytpS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/438 NBRP B. subtilis, Japan]
1A984 ( ''sftA''::''spec''), [Pubmed|19788545], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A984&Search=1A984 BGSC]
BKE29805 (''[gene|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]''::''erm'', available in the BGSC, in [wiki|Fabian Commichau]'s, and in [wiki|Jörg Stülke]'s lab) [pubmed|28189581]
BKE29805 (Δ[gene|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29805 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTTATCTCTAACTCC, downstream forward: _UP4_TAATATGGGTTCATTTTCAC
BKK29805 (Δ[gene|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29805 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTTATCTCTAACTCC, downstream forward: _UP4_TAATATGGGTTCATTTTCAC
References
Reviews
Original Publications
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Time of last update: 2024-12-22 02:52:09
Author of last update: Jstuelk