ysxC
168
assembly of the 50S subunit of the ribosome
locus
BSU_28190
Molecular weight
21.88 kDa
pI
9.71
function
ribosome assembly
product
GTPase
essential
yes
synonyms
ysxC, orfX, engB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0218 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,879,882 → 2,880,469
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
binds and hydrolyzes GTP and readily exchanges GDP for GTP
participates in the maturation of the central protuberance, GTPase associated region and key RNA helices in the A, P and E functional sites of the 50S subunit [Pubmed|27484475]
Protein family
[wiki|TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily] (according to UniProt)
[wiki|Domains]
EngB-type G (aa 22-195) (according to UniProt)
Structure
[PDB|1SVI] (complex with GDP) [pubmed|15136032]
[PDB|1SUL] [pubmed|15136032]
[AF|P38424]
Paralogous protein(s)
[protein|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA], [protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF], [protein|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg], [protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH], [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA], [protein|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|engA], [protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF], [protein|CB68B3393CA7862C45C624AF7B1ADD188243A01F|era]
[wiki|Localization]
cytosol (according to UniProt)
Expression and Regulation
Operons
genes
[gene|FF15BE26BCC78EC1301C58A51AF0A519D7BE9ADC|lonA]-[gene|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|ysxC]
description
[Pubmed|7961402]
regulation
induced by heat stress ([protein|search|CtsR]) [Pubmed|9852015]
regulatory mechanism
[protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR]: repression, [Pubmed|9852015], in [regulon|protein:908DB17A39D518E84977250C55825E77FA02E391|ctsR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7961402], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A996 (ysxC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/996 NBRP B. subtilis, Japan]
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP834, available in [wiki|Stülke] lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP842, available in [wiki|Stülke] lab
labs
[wiki|Naotake Ogasawara], Nara, Japan
References
Reviews
Original Publications
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Time of last update: 2024-10-09 13:18:25
Author of last update: Jstuelk