fadF
168
similar to iron-sulphur-binding reductase
locus
BSU_37180
Molecular weight
79.00 kDa
pI
6.53
function
fatty acid degradation
product
unknown
essential
no
synonyms
fadF, ywjF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0247 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,814,382 → 3,816,499
The protein
[wiki|Domains]
2 [wiki|4Fe-4S ferredoxin-type domain]s (aa 268-298, aa 360-391) (according to UniProt)
[wiki|Cofactors]
2 4Fe-4S clusters [pubmed|29292548]
Structure
[AF|P45866]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|DEE0DA15EE9EA27BA8C332204E40D336324A111C|fadF]-[gene|7D89BBB52403BF99416250688EFA90C2BE8EB591|acdA]-[gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]
description
[Pubmed|17189250]
regulation
repressed in the absence of long-chain fatty acids ([protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]) [Pubmed|17189250]
regulatory mechanism
[protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]: repression, [Pubmed|17189250], in [regulon|protein:8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|17189250], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A555 (ywjF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/555 NBRP B. subtilis, Japan]
BKE37180 (''[gene|DEE0DA15EE9EA27BA8C332204E40D336324A111C|fadF]''::''erm'', available in the BGSC, in [wiki|Fabian Commichau]'s, and in [wiki|Jörg Stülke]'s lab) [pubmed|28189581]
BKE37180 (Δ[gene|DEE0DA15EE9EA27BA8C332204E40D336324A111C|fadF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCAATTCCCCCTTTTT, downstream forward: _UP4_GATCTGAAAATGGGGGAGAA
BKK37180 (Δ[gene|DEE0DA15EE9EA27BA8C332204E40D336324A111C|fadF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCAATTCCCCCTTTTT, downstream forward: _UP4_GATCTGAAAATGGGGGAGAA
References
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Time of last update: 2024-12-22 07:09:56
Author of last update: Melvin.boenninger