amiE
168
N-acetylmuramyl-L-alanine amidase
locus
BSU_01670
Molecular weight
49.14 kDa
pI
9.35
function
recycling of cell wall material
product
N-acetylmuramyl-L-alanine amidase
essential
no
synonyms
amiE, ybbE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1680 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
188,408 → 189,733
The protein
Catalyzed reaction/ biological activity
hydrolyzes the amide bond between MurNAc and the L-alanine residue of the stem peptide [Pubmed|20400549]
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
Protein family
peptidase S12 family (single member, according to UniProt)
Structure
[PDB|4IVK] (from uncultured bacterium, 25% identity) [pubmed|23737193]
[AF|O05213]
Expression and Regulation
Operons
genes
[gene|0B1F43F43A4DC1574A153DBFBAA5D0A1855F9B61|murQ]-[gene|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]-[gene|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]-[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]-[gene|A5D4BF262BD365DCEBA9358C8DB295E605DE4D84|nagZ]-[gene|005D37F34CA3D6891F7D8FF7A2AFA1CBCB556FFE|namZ]
description
[Pubmed|22383849]
regulation
expressed in late exponential and early stationary phase [Pubmed|20400549]
regulatory mechanism
[protein|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]: repression, [pubmed|30038046], in [regulon|protein:125872506C400CB6B3DAEA8B5130B064DFBA4494|murR regulon]
Biological materials
Mutant
BKE01670 (Δ[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGGCTAGCCCCTTTCTG, downstream forward: _UP4_TGAATGATTAGACGATAGGA
BKK01670 (Δ[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGGCTAGCCCCTTTCTG, downstream forward: _UP4_TGAATGATTAGACGATAGGA
References
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Time of last update: 2024-12-19 19:55:36
Author of last update: Melvin.boenninger