amyE
168
alpha-amylase
locus
BSU_03040
Molecular weight
72.62 kDa
pI
5.85
function
starch degradation
product
alpha-amylase
essential
no
ec
3.2.1.1
synonyms
amyE, amyA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0366 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
327,618 → 329,597
The protein
Catalyzed reaction/ biological activity
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units (according to UniProt)
Protein family
[wiki|glycosyl hydrolase 13 family] (according to UniProt)
Structure
[PDB|1BAG] (complex with maltopentaose)
[AF|P00691]
[wiki|Localization]
secreted (according to Swiss-Prot), extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|E48DE0C73CB859E2B367B6800E85BD94836390A5|ycgB]-[gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]
description
[Pubmed|22383849]
genes
[gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]
description
[Pubmed|11320136]
regulation
repressed by glucose ([protein|search|CcpA]) [Pubmed|7665492]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|7665492], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: activation, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|3123701], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP550 (cat), available in [wiki|Stülke] lab
BKE03040 (Δ[gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTGACACTCCTTATT, downstream forward: _UP4_TGAGGGCAAGGCTAGACGGG
BKK03040 (Δ[gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTGACACTCCTTATT, downstream forward: _UP4_TGAGGGCAAGGCTAGACGGG
References
Reviews
Original Publications
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Time of last update: 2025-01-05 05:35:17
Author of last update: Melvin.boenninger