thiL
168
thiamine-monophosphate kinase
locus
BSU_05900
Molecular weight
35.73 kDa
pI
5.04
function
biosynthesis of thiamine
product
thiamine-monophosphate kinase
essential
no
ec
2.7.4.16
synonyms
thiL, ydiA, ydxA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0611 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
640,662 → 641,639
The protein
Catalyzed reaction/ biological activity
ATP + thiamine phosphate --> ADP + thiamine diphosphate (according to UniProt)
Protein family
thiamine-monophosphate kinase family (single member, according to UniProt)
Structure
[PDB|1VQV] (from ''Aquifex aeolicus'', 33% similarity, 51% identity)
[AF|O05514]
Expression and Regulation
Operons
genes
[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]-[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]-[gene|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|rimI]-[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-C197 (ydiA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2195 NBRP B. subtilis, Japan]
BKE05900 (Δ[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACCAAAACCTCCTTT, downstream forward: _UP4_TTTAAATAAGACGGGTGACT
BKK05900 (Δ[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACCAAAACCTCCTTT, downstream forward: _UP4_TTTAAATAAGACGGGTGACT
References
Reviews
Original Publications
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Time of last update: 2025-01-03 09:55:53
Author of last update: Melvin.boenninger