menH
168
menaquinone biosynthesis methyltransferase
locus
BSU_22750
Molecular weight
26.43 kDa
pI
8.17
function
biosynthesis of menaquinone
product
menaquinone biosynthesis methyltransferase
essential
no
ec
2.1.1.163
synonyms
menH, gerCB, menG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2226 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,382,907 → 2,383,608
Phenotypes of a mutant
essential [http://www.ncbi.nlm.nih.gov/pubmed/?term=12682299 according to Kobayashi et al.]; however, inactivation reported by [http://www.ncbi.nlm.nih.gov/pubmed/?term=26735940 Meeske et al.] and [http://www.ncbi.nlm.nih.gov/pubmed/?term=20511502 Block et al.]
inactivation of ''[gene|DDD40AEBBCBD3C19661A72B85912E266DBF267B3|menH]'' reduces sporulation efficiency to 12.7% that of wild type cells [Pubmed|26735940]
defective in biofilm formation [pubmed|31420537]
The protein
Catalyzed reaction/ biological activity
2-demethylmenaquinol + S-adenosyl-L-methionine --> menaquinol + H+ + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
[wiki|Methyltransferase superfamily] (according to UniProt)
[wiki|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)
[wiki|Domains]
[wiki|AB hydrolase-1 domain] (aa 26-259) (according to InterPro)
Structure
[PDB|1OBW] (from ''Saccharomyces cerevisiae'', 31% identity) [Pubmed|9201917]
[AF|P31113]
Expression and Regulation
Operons
genes
[gene|C45A5935559F0DAAD70F808725AF730BC18A2B0A|hepS]-[gene|DDD40AEBBCBD3C19661A72B85912E266DBF267B3|menH]-[gene|3CAEA54E80A0B56EE120B881C8CDBE055AF6BDC1|hepT]-[gene|B498ED03F19B7A73878D5C947C28C2A87CAB934D|ndk]
description
[pubmed|22383849]
additional information
highly expressed at high iron concentrations [pubmed|31420537]
Biological materials
Mutant
BKE22750 (Δ[gene|DDD40AEBBCBD3C19661A72B85912E266DBF267B3|menH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCTTTTGAGTCCTGCATAA, downstream forward: _UP4_CTGTTTGAAGAGGCGGGCCT
BKK22750 (Δ[gene|DDD40AEBBCBD3C19661A72B85912E266DBF267B3|menH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCTTTTGAGTCCTGCATAA, downstream forward: _UP4_CTGTTTGAAGAGGCGGGCCT
References
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Time of last update: 2025-01-30 19:03:53
Author of last update: Melvin.boenninger