metS
168
methionyl-tRNA synthetase
locus
BSU_00380
Molecular weight
76.01 kDa
pI
4.96
function
translation
product
methionyl-tRNA synthetase
essential
yes
ec
6.1.1.10
synonyms
metS, metG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0143 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
45,633 → 47,627
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
ATP + L-methionine + tRNAMet --> AMP + diphosphate + L-methionyl-tRNAMet (according to UniProt)
Protein family
[wiki|Class-I aminoacyl-tRNA synthetase family] (according to UniProt)
[wiki|Domains]
[wiki|tRNA-binding domain] (aa 570-662) (accordinig to UniProt)
Structure
[PDB|1WE0] (from ''Thermus thermophilus hb8 mutant'', 40% identity, 57% similarity) [Pubmed|10673435]
[AF|P37465]
Modification
phosphorylated on Ser-652 [Pubmed|20509597]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|DCBDD135F295B04FE7C7EB92E94638701CB320DD|metS]-[gene|00798BB5A769C464CC5FDDF7D9E41D30B473B9A8|yabD]
description
[Pubmed|22383849] null
regulation
repressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
additional information
term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of [gene|DCBDD135F295B04FE7C7EB92E94638701CB320DD|metS] [Pubmed|27120414]
genes
[gene|DCBDD135F295B04FE7C7EB92E94638701CB320DD|metS]
description
null
regulation
repressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
additional information
term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of [gene|DCBDD135F295B04FE7C7EB92E94638701CB320DD|metS] [Pubmed|27120414]
References
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Time of last update: 2024-12-30 06:55:27
Author of last update: Robert.warneke