holB
168
DNA polymerase III (delta subunit), part of the clamp-loader complex and the [wiki|replisome]
locus
BSU_00310
Molecular weight
37.47 kDa
pI
6.33
function
[category|SW.3.1.1|DNA replication]
product
DNA polymerase III (delta subunit)
essential
yes
ec
2.7.7.7
synonyms
holB, yaaS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0470 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
40,665 → 41,654
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
required for bacteriophage SPP1 replication [Pubmed|23268446]
Structure
[PDB|1NJG] (from ''E. coli'', 36% identity) [Pubmed|12623013]
[AF|P37540]
[wiki|Localization]
forms foci [Pubmed|21958350]
cytoplasm (homogeneous) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|08314FA6195D553B8522BCD3B45FEE0EAB51F64D|xpaC]-[gene|5231A0EE9C5A7556817B686D5B2B7B72B675BCAC|yaaN]-[gene|EEF3E572ED2F23581EB9B48D16B3112B886F7975|yaaO]-[gene|5B9984BBCEC1244628DC1467BE26976AD8E06286|tmk]-[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|trmN6]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
description
[Pubmed|22383849]
genes
[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|trmN6]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
description
[Pubmed|22383849]
regulation
expressed during growth and the transition phase, expression is erduced in stationary phase [Pubmed|23490197]
genes
[gene|5B9984BBCEC1244628DC1467BE26976AD8E06286|tmk]-[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|trmN6]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
description
[Pubmed|22383849]
Biological materials
Expression vectors
pGP1692: expression of Strep-''holB''-Strep in ''E. coli'', in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
pGP1698: expression of ''holB''-Strep in ''E. coli'', in [wiki|pGP574], available in [wiki|Jörg Stülke]'s lab
pGP1699: expression of ''holB'' in ''E. coli'', in [wiki|pGP574] (no tag due to a stop codon at the end of ''holB''), available in [wiki|Jörg Stülke]'s lab
pGP2672: expression of Strep-''holB'' by [wiki|pGP380] in ''B. subtilis'', suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP3028: expression of ''holB''-Strep by [wiki|pGP382] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
Page visits: 8083
Time of last update: 2024-10-12 22:06:22
Author of last update: Jstuelk