sigH

sigH
168

[wiki|RNA polymerase] [wiki|sigma factor] SigH, not fully active in laboratory strains due to a mutation (V117A)

locus
BSU_00980
Molecular weight
25.30 kDa
pI
5.15
Protein length
218 aaSequenceBlast
Gene length
657 bpSequenceBlast
function
transcription of early stationary phase genes ([wiki|sporulation], competence)
product
[wiki|RNA polymerase] [wiki|sigma factor] SigH
essential
no
synonyms
sigH, spo0H
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1595 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
116,600 → 117,256
The protein
Protein family
[wiki|Sigma-70 factor family] (according to UniProt)
[wiki|Domains]
[wiki|HTH luxR-type domain] (aa 179-206) (according to InterPro)
Structure
[PDB|3MZY] (from Fusobacterium nucleatum, 40% identity)
[AF|P17869]
Additional information
not fully active in laboratory strains due to a mutation (V117A) [pubmed|27501195]
the poor interaction of [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH] with the [wiki|RNA polymerase] is responsible for the poor [wiki|sporulation] at high salinity, this phenotype can be partially suppressed by specific mutations in [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH] [Pubmed|26712348]
Expression and Regulation
Operons
genes
[gene|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]-[gene|BBD82B93D5E880AB7093ED53DDB149B0E0B3BAE4|rpmGB]-[gene|1007EA15FB7AF81A44400287DB68BCC497158ACF|secE]
description
[Pubmed|22383849]
regulation
repressed during logrithmic growth ([protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]) [Pubmed|7592498]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|7592498], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1898930], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]→[gene|1007EA15FB7AF81A44400287DB68BCC497158ACF|secE]

2024-12-03 07:23:11

Jstuelk

129

c2494a3b727f37ab2c96e1e6e5100f53e9bfaefe

A55AB4CD7536D144E751A14E2E3E513DF4E48707

Biological materials
Mutant
BKE00980 (Δ[gene|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00980 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTCCGATCCCCCCGGCGC,  downstream forward: _UP4_TAATAGGAATTTATGCTATA
BKK00980 (Δ[gene|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00980 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTCCGATCCCCCCGGCGC,  downstream forward: _UP4_TAATAGGAATTTATGCTATA
labs
[wiki|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]
References
Reviews
20833318,35638784
Original Publications
36815589,1391042,3123466,2122453,3127379,2502532,6405278,2118512,2509422,1904128,3007003,2500529,9733708,11377871,8600030,7592498,1898930,2509423,20154131,26712348,28507241,27501195,29363854,37380492,39427753
The [wiki|SigH regulon]
12169614

DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4

Page visits: 9008

Time of last update: 2024-12-03 15:34:46

Author of last update: Jstuelk