wprA
168
secreted quality control protease
locus
BSU_10770
Molecular weight
96.29 kDa
pI
9.58
function
protein quality control
product
secreted quality control protease
essential
no
synonyms
wprA, yisM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1404 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,153,789 → 1,156,473
The protein
Catalyzed reaction/ biological activity
important for [protein|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY]-[protein|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY]-dependent export of [protein|253A24EEA02C07E577A96FB64DF39F9A3D0C3A52|ykuE] and [protein|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB] [Pubmed|23180473]
involved in degradation of [protein|6EE13E61A218A6D52BAC83A1145B0B96961CE289|prsA], [protein|76FA52D5A5F9FCA14BA9B45872FE3D99D89B211B|htrA], and [protein|796216BE9CD6DADFEADC29497253C81BF496A2ED|htrB] [Pubmed|24362423]
Protein family
[wiki|peptidase S8 family] (according to UniProt)
[wiki|Domains]
[wiki|peptidase S8 domain] (aa 422-729) (according to UniProt)
Structure
[PDB|1DBI] (the peptidase S8 domain from Bacillus sp., aa 458 ... 687, 46% identity) [pubmed|10588904]
[AF|P54423]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862]
membrane [Pubmed|18763711]
cell wall [Pubmed|23180473]
Expression and Regulation
Operons
genes
[gene|DC0FDCA3FA6742B023E6877CE9554AA1D47012BB|wprA]
description
[Pubmed|16306698]
regulation
repressed by glucose ([protein|search|CcpA]) [Pubmed|22900538]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|22900538], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb]: activation, [Pubmed|16306698], in [regulon|protein:7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22900538], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
An [wiki|ncRNA] is predicted for'[protein|search|wprA]' [PubMed|20525796]
Biological materials
Mutant
1A1021 ( ''wprA''::''kan''), [Pubmed|20400548], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1021&Search=1A1021 BGSC]
BKE10770 (Δ[gene|DC0FDCA3FA6742B023E6877CE9554AA1D47012BB|wprA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATCCCTCCTGCAAA, downstream forward: _UP4_TAACCAAAAAGCGGTGCTCG
BKK10770 (Δ[gene|DC0FDCA3FA6742B023E6877CE9554AA1D47012BB|wprA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATCCCTCCTGCAAA, downstream forward: _UP4_TAACCAAAAAGCGGTGCTCG
References
Reviews
Original Publications
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Time of last update: 2024-12-21 15:48:10
Author of last update: Jstuelk