ganA
168
beta-galactosidase
locus
BSU_34130
Molecular weight
79.08 kDa
pI
5.7
function
galactan utilization
product
beta-galactosidase
essential
no
ec
3.2.1.23
synonyms
ganA, yvfN, lacA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1874 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,503,020 → 3,505,083
Phenotypes of a mutant
a ''[gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]-[gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]-[gene|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|ganQ]-[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]-[gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]'' mutant is impaired in the utilization of galactan [Pubmed|22893383]
The protein
Catalyzed reaction/ biological activity
degradation of galactotriose to galactose [Pubmed|22893383]
degradation of ß-1.4-galactobiose to galactose [Pubmed|28617843]
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides (according to UniProt)
Protein family
glycosyl hydrolase 42 family (with [protein|3226901F1D7C1F21296AB974CAE3656C18BE8F69|yesZ], according to UniProt)
Structure
[PDB|4OJY] (from Geobacillus stearothermophilus, 76% identity) [pubmed|24100561]
[AF|O07012]
Modification
phosphorylated on Arg-387 [Pubmed|22517742]
Effectors of protein activity
inhibited by galactose [pubmed|28617843]
Expression and Regulation
Operons
genes
[gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]-[gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]-[gene|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|ganQ]-[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]-[gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]
description
[Pubmed|9287030]
regulation
induced by galactan (binding of galactobiose to [protein|1F787F70C87DEB221709579122D63C2322A84E56|ganR]) [Pubmed|28617843,27501980]
repressed by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|28617843,27501980]
regulatory mechanism
[protein|1F787F70C87DEB221709579122D63C2322A84E56|ganR]: repression, [Pubmed|9287030], in [regulon|protein:1F787F70C87DEB221709579122D63C2322A84E56|ganR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [pubmed|28617843], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|27501980], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP1181 (aphA3) available in [wiki|Jörg Stülke]'s lab
GP2147 (phleo) available in [wiki|Jörg Stülke]'s lab
1A785 ( ''ganA''::''spec''), [Pubmed|11274134], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A785&Search=1A785 BGSC]
1A786 ( ''ganA''::''spec''), [Pubmed|11274134], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A786&Search=1A786 BGSC]
BKE34130 (Δ[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACATTCTCCTCCTTGT, downstream forward: _UP4_TAGGCTGATGCTCCGCTCGA
BKK34130 (Δ[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACATTCTCCTCCTTGT, downstream forward: _UP4_TAGGCTGATGCTCCGCTCGA
References
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Time of last update: 2024-10-07 16:43:39
Author of last update: Melvin.boenninger