yhcR
168
extracellular non-specific nuclease, degrades DNA in the biofilm to release phosphate ions
locus
BSU_09190
Molecular weight
132.47 kDa
pI
4.69
function
utilization of nucleic acids in biofilms
product
extracellular non-specific nuclease, DNase
essential
no
ec
null
synonyms
yhcR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4085 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
991,348 → 995,001
The protein
Catalyzed reaction/ biological activity
degradation of extracellular DNA in biofilms to obtain phosphate ions [pubmed|39375363]
Protein family
5'-nucleotidase family (with [protein|B791B0603042603CB5B5E03CA129B20D02BC02BC|yfkN], according to UniProt)
[wiki|Domains]
TNase-like domain (aa 376-517) (according to UniProt)
[wiki|Cofactors]
Ca(2+) [pubmed|39375363]
Structure
[PDB|2Z1A] (from Thermus thermophilus, corresponds to aa 590 ... 1094, 29% identity)
[AF|P54602]
Paralogous protein(s)
[protein|B791B0603042603CB5B5E03CA129B20D02BC02BC|yfkN]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862], attached to the cell wall by sorties [protein|DAFBC470DA0FA437B117716DE899A8D3EC454672|yhcR] [Pubmed|21800427]
Expression and Regulation
Operons
genes
[gene|DAFBC470DA0FA437B117716DE899A8D3EC454672|yhcR]-[gene|A24243129860F1B043EBE1358FAEA57BED111BBC|yhcS]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-A665 (yhcR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/665 NBRP B. subtilis, Japan]
BKE09190 (Δ[gene|DAFBC470DA0FA437B117716DE899A8D3EC454672|yhcR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAATTCCTTTCAGGCT, downstream forward: _UP4_AGAAAAAGGAGCGCCTCCAG
BKK09190 (Δ[gene|DAFBC470DA0FA437B117716DE899A8D3EC454672|yhcR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAATTCCTTTCAGGCT, downstream forward: _UP4_AGAAAAAGGAGCGCCTCCAG
labs
[wiki|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers%20Laboratories%20and%20Programs/Bechhofer%20Laboratory?citype=Physician&ciid=Bechhofer%20David%20H%201255565 Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-20 14:48:36
Author of last update: Jstuelk