ackA
168
acetate kinase
locus
BSU_29470
Molecular weight
42.98 kDa
pI
5.19
function
overflow metabolism
product
acetate kinase
essential
no
ec
2.7.2.1
synonyms
ackA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0282 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,015,111 3,016,298
The protein
Catalyzed reaction/ biological activity
acetate + ATP --> acetyl phosphate + ADP (according to UniProt)
Protein family
acetokinase family (with [protein|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk], according to UniProt)
Structure
[PDB|2IIR] (from ''Thermotoga maritima'', 54% identity, 73% similarity)
[AF|P37877]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|DAA285C86692E8B47D48652E0973AE3FF091CBC3|ackA]
description
[Pubmed|8226682]
regulation
activated during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|16995897]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: activation, [Pubmed|16995897], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: activation, [Pubmed|8226682,12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8226682], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP1902 (''ackA''::''aphA3'') (available in [wiki|Jrg Stlke]'s lab)
BKE29470 ([gene|DAA285C86692E8B47D48652E0973AE3FF091CBC3|ackA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTGACGCTCCTTTAT, downstream forward: _UP4_TAAATCGCATGAAAGCACAT
BKK29470 ([gene|DAA285C86692E8B47D48652E0973AE3FF091CBC3|ackA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTGACGCTCCTTTAT, downstream forward: _UP4_TAAATCGCATGAAAGCACAT
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jrg Stlke]'s lab
labs
[wiki|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-21 20:59:07
Author of last update: Jstuelk