ycsE
168
5'-nucleotidase, dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate
locus
BSU_04040
Molecular weight
27.92 kDa
pI
4.49
function
dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate
product
5'-nucleotidase
essential
no
synonyms
ycsE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0561 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
456,068 → 456,817
The protein
Catalyzed reaction/ biological activity
dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate [Pubmed|26316208]
5-amino-6-(5-phospho-D-ribitylamino)uracil + H2O --> 5-amino-6-(D-ribitylamino)uracil + phosphate (according to UniProt)
Protein family
[wiki|HAD superfamily] (according to UniProt) [Pubmed|26316208]
[wiki|Cof family] (according to UniProt)
Structure
[PDB|1NRW] ([protein|AF9523D3AA3C032B70175BCFFED34E9A42ED01AF|phoC], 31% identity)
[AF|P42962]
Expression and Regulation
Operons
genes
[gene|D8AC0ECC57C6113D0CCA36FC38395EECBD00FF50|ycsE]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-C071 (ycsE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2069 NBRP B. subtilis, Japan]
BKE04040 (Δ[gene|D8AC0ECC57C6113D0CCA36FC38395EECBD00FF50|ycsE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATGTACCTCTCTTTA, downstream forward: _UP4_TAAAAAAAGAGAGTCCTAAG
BKK04040 (Δ[gene|D8AC0ECC57C6113D0CCA36FC38395EECBD00FF50|ycsE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATGTACCTCTCTTTA, downstream forward: _UP4_TAAAAAAAGAGAGTCCTAAG
References
Page visits: 3010
Time of last update: 2024-12-30 14:48:44
Author of last update: Jstuelk