khtT

khtT
168

K+/H+ antiporter for K+ efflux

locus
BSU_09860
Molecular weight
18.59 kDa
pI
4.89
Protein length
165 aaSequenceBlast
Gene length
498 bpSequenceBlast
function
potassium ion efflux
product
K+/H+ antiporter for K+ efflux
essential
no
synonyms
khtT, yhaT
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0490 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,060,427 → 1,060,924
The protein
[wiki|Domains]
contains a [wiki|RCK_C domain] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=RCK+C-terminal+domain&sort=score x])
Structure
[PDB|7AGV] (complex with c-di-AMP) [pubmed|33790011]
[AF|O07535]
Effectors of protein activity
binds c-di-AMP, which activates export activity [pubmed|33790011,31061098]
Paralogous protein(s)
[protein|4AF865F1E16187F89C1FE19BAE5C2CF2C0A53E4A|yrvC]
[wiki|Localization]
cell membrane (peripheral) (according to UniProt)
Expression and Regulation
Operons
genes
[gene|A54E8E38AEF596D09B4BEB64D9F539A8AD34E73D|khtS]-[gene|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT]-[gene|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU]
description
[Pubmed|22383849]
Open in new tab

[gene|A54E8E38AEF596D09B4BEB64D9F539A8AD34E73D|khtS]→[gene|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU]

2024-10-07 16:34:46

ghost

100

54fb18209326e1c8d64853667143b4fd8e7337e1

ACAE2230F2FD54981F6B24985DF9C660B7B5FFBB

Biological materials
Mutant
MGNA-A675 (yhaT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/675 NBRP B. subtilis, Japan]
GP2078 (Δ[gene|A54E8E38AEF596D09B4BEB64D9F539A8AD34E73D|khtS]-[gene|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT]-[gene|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU]::''aphA3''), available in [wiki|Jörg Stülke]'s lab
BKE09860 (Δ[gene|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGAAAATCCCTCCGAAT,  downstream forward: _UP4_TCTGGAGAGGGCGTGTGAGC
BKK09860 (Δ[gene|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGAAAATCCCTCCGAAT,  downstream forward: _UP4_TCTGGAGAGGGCGTGTGAGC
Expression vectors
pGP2906 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
27935846,31361596,32603625
Original Publications
14987767,17679694,24330391,31061098,33790011

D8A8FBC05B9665F72A88685D2B33822C2BB84C62

Page visits: 3454

Time of last update: 2024-10-13 19:19:04

Author of last update: Jstuelk