epeX
168
competition determinant, secreted linear antimicrobial peptide, controls [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]-[protein|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS] activity
locus
BSU_40180
Molecular weight
5.65 kDa
pI
9.57
function
conpetition with other bacteria, control of [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]-[protein|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS] activity
product
secreted antimicrobial peptide
essential
no
synonyms
yydF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
4,127,498 → 4,127,647
The protein
Structure
[AF|Q45596]
Modification
modified (epimerization of Val-36 and Ile-44 to the D-forms) by the radical SAM epimerase [protein|25FC87037222084ABE318813959101016FEB49DF|epeE] [Pubmed|28644475,23106164]
processing to the mature modified peptide (epipeptide) by the membrane protease [protein|E280A5994F4D9D93DA4B698E79C86360DBEE5396|epeP] [pubmed|28644475]
[wiki|Localization]
extracellular (processing requires [protein|E280A5994F4D9D93DA4B698E79C86360DBEE5396|epeP], transport by [protein|1BF1A48CF645249CA9ECBB19C04B059B11D821EF|epeA]-[protein|A4213DE6C9BE29405B0A4C0A969A78EA4C5EB71F|epeB]) [Pubmed|23106164]
Expression and Regulation
Operons
genes
[gene|D66B9BFF79ADD8B0F9BFA3892FCF4597264CE9EE|epeX]-[gene|25FC87037222084ABE318813959101016FEB49DF|epeE]-[gene|E280A5994F4D9D93DA4B698E79C86360DBEE5396|epeP]-[gene|1BF1A48CF645249CA9ECBB19C04B059B11D821EF|epeA]-[gene|A4213DE6C9BE29405B0A4C0A969A78EA4C5EB71F|epeB]
description
[Pubmed|17921301]
regulation
repressed during logarithmic growth ([protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]) [Pubmed|17921301]
expression
expression is heterogeneous [pubmed|35171018]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|34120110,17921301], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]: repression, [Pubmed|15743949], in [regulon|protein:1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|17921301], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is very stable (> 15 min) [pubmed|12884008]
the [gene|D66B9BFF79ADD8B0F9BFA3892FCF4597264CE9EE|epeX] portion of the mRNA belongs to the most stable mRNAs in B. subtilis [pubmed|17921301]
Biological materials
Mutant
BKE40180 (Δ[gene|D66B9BFF79ADD8B0F9BFA3892FCF4597264CE9EE|epeX]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATCCCTCCTCCTTT, downstream forward: _UP4_TAATTAGAGGGGTACAAAGG
BKK40180 (Δ[gene|D66B9BFF79ADD8B0F9BFA3892FCF4597264CE9EE|epeX]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATCCCTCCTCCTTT, downstream forward: _UP4_TAATTAGAGGGGTACAAAGG
References
Reviews
Original Publications
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Time of last update: 2024-12-21 01:31:41
Author of last update: Jstuelk