epeX

epeX
168

competition determinant, secreted linear antimicrobial peptide, controls [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]-[protein|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS] activity

locus
BSU_40180
Molecular weight
5.65 kDa
pI
9.57
Protein length
Gene length
function
conpetition with other bacteria, control of [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]-[protein|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS] activity
product
secreted antimicrobial peptide
essential
no
synonyms
yydF

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
4,127,498 → 4,127,647
The protein
Structure
[AF|Q45596]
Modification
modified (epimerization of Val-36 and Ile-44 to the D-forms) by the radical SAM epimerase [protein|25FC87037222084ABE318813959101016FEB49DF|epeE] [Pubmed|28644475,23106164]
processing to the mature modified peptide (epipeptide) by the membrane protease [protein|E280A5994F4D9D93DA4B698E79C86360DBEE5396|epeP] [pubmed|28644475]
[wiki|Localization]
extracellular (processing requires [protein|E280A5994F4D9D93DA4B698E79C86360DBEE5396|epeP], transport by [protein|1BF1A48CF645249CA9ECBB19C04B059B11D821EF|epeA]-[protein|A4213DE6C9BE29405B0A4C0A969A78EA4C5EB71F|epeB]) [Pubmed|23106164]
Expression and Regulation
Operons
genes
[gene|D66B9BFF79ADD8B0F9BFA3892FCF4597264CE9EE|epeX]-[gene|25FC87037222084ABE318813959101016FEB49DF|epeE]-[gene|E280A5994F4D9D93DA4B698E79C86360DBEE5396|epeP]-[gene|1BF1A48CF645249CA9ECBB19C04B059B11D821EF|epeA]-[gene|A4213DE6C9BE29405B0A4C0A969A78EA4C5EB71F|epeB]
description
[Pubmed|17921301]
regulation
repressed during logarithmic growth ([protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]) [Pubmed|17921301]
expression
expression is heterogeneous [pubmed|35171018]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|34120110,17921301], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]: repression, [Pubmed|15743949], in [regulon|protein:1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|17921301], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|D66B9BFF79ADD8B0F9BFA3892FCF4597264CE9EE|epeX]→[gene|A4213DE6C9BE29405B0A4C0A969A78EA4C5EB71F|epeB]

2024-10-08 00:28:43

Jstuelk

84

078981D5A4F256B0FA27157DFABCDDE534EC3306

BE1247045A413B93C3150C913C43B4E1B8098926

additional information
the mRNA is very stable (> 15 min) [pubmed|12884008]
the [gene|D66B9BFF79ADD8B0F9BFA3892FCF4597264CE9EE|epeX] portion of the mRNA belongs to the most stable mRNAs in B. subtilis [pubmed|17921301]
Biological materials
Mutant
BKE40180 (Δ[gene|D66B9BFF79ADD8B0F9BFA3892FCF4597264CE9EE|epeX]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTATCCCTCCTCCTTT,  downstream forward: _UP4_TAATTAGAGGGGTACAAAGG
BKK40180 (Δ[gene|D66B9BFF79ADD8B0F9BFA3892FCF4597264CE9EE|epeX]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTATCCCTCCTCCTTT,  downstream forward: _UP4_TAATTAGAGGGGTACAAAGG
References
Reviews
23106164
Original Publications
12884008,17921301,17921301,21815947,23199363,28644475,32117169,34120110,37289492,36815589

D66B9BFF79ADD8B0F9BFA3892FCF4597264CE9EE

Page visits: 4712

Time of last update: 2024-10-08 02:31:08

Author of last update: Jstuelk