cwlH
168
N-acetylmuramoyl-L-alanine amidase
locus
BSU_25710
Molecular weight
27.42 kDa
pI
10
function
cell wall metabolism
product
N-acetylmuramoyl-L-alanine amidase
essential
no
ec
3.5.1.28
synonyms
cwlH, yqeE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5632 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,648,903 → 2,649,655
The protein
Catalyzed reaction/ biological activity
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)
Protein family
[wiki|N-acetylmuramoyl-L-alanine amidase 2 family] (according to UniProt)
[wiki|Domains]
contains an amidase_2 domain (like [protein|C1EB8D05386C1B35B8002239F5247CC6AB25F786|blyA], [protein|C9C395A5F6A2C06EAC97F228C5C66D1628C937CF|cwlA], [protein|50D7B97E4D3D41F74EF1942B85DE1DA4A33BEBB0|xlyA], [protein|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|xlyB])
[wiki|N-acetylmuramoyl-L-alanine amidase domain] (aa 45-141) (according to UniProt)
Structure
[PDB|3RDR] (N-terminal domain, aa 1 - 150, XlyA, 75% identity) [pubmed|21816821]
[PDB|1LBU] (C-terminal peptidoglycan-binding domain, aa 183 - 245, 48% identity)
[AF|P54450]
Paralogous protein(s)
[protein|50D7B97E4D3D41F74EF1942B85DE1DA4A33BEBB0|xlyA], [protein|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|xlyB], [protein|C9C395A5F6A2C06EAC97F228C5C66D1628C937CF|cwlA]
[wiki|Localization]
secreted (according to UniProt)
Expression and Regulation
Operons
genes
[gene|D5612882F1887BE342EF7072E71502382AB7289F|cwlH]
description
[Pubmed|10515909]
regulation
expressed late during sporulation in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|10515909]
regulatory mechanism
[protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: activation, [Pubmed|10515909], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|10515909], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
Biological materials
Mutant
BKE25710 (Δ[gene|D5612882F1887BE342EF7072E71502382AB7289F|cwlH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATCCCTCCCTATT, downstream forward: _UP4_TAAATGCAGGATTTAACGGA
BKK25710 (Δ[gene|D5612882F1887BE342EF7072E71502382AB7289F|cwlH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATCCCTCCCTATT, downstream forward: _UP4_TAAATGCAGGATTTAACGGA
References
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Time of last update: 2024-12-20 16:51:25
Author of last update: Melvin.boenninger