cotH
168
protein kinase, required for the assembly of several outer coat proteins
locus
BSU_36060
Molecular weight
42.65 kDa
pI
5.95
function
protection of [protein|EB690FA075EAC3E298C47AED082B5FC21E8F8F46|cotU] and [protein|9C59B51FC19FC83BD14272008BD441833DD1738E|cotC] in the mother cell
product
protein kinase
essential
no
synonyms
cotH, ywrH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5337 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,716,009 → 3,717,097
Phenotypes of a mutant
overexpression of ''cotH'' suppresses the phenotypes of a [gene|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE] mutant [Pubmed|24086406]
reduced [wiki|germination] efficiency [Pubmed|27185916]
The protein
Catalyzed reaction/ biological activity
autophosphorylation with ATP and subsequent phosphorylation of [protein|2A0B2CA7C982C03C1AFBDB3540A918EE937B06CB|cotB] and [protein|49BAFE3E69F1F91AEF4154F55B44639DD3CE4A71|cotG] on serine residues [Pubmed|32592201,27185916]
Protein family
cotH family (with [protein|75FBA935A39AF67DB4E69C8699D29D9F5C149143|yisJ], according to UniProt)
[wiki|Cofactors]
Mn2+ [Pubmed|27185916]
Structure
[PDB|5JD9] [Pubmed|27185916]
[AF|Q45535]
[wiki|Localization]
inner spore coat
Expression and Regulation
Operons
genes
[gene|D53067098F693669F61E5CCF6C06664580C40C79|cotH]-[gene|2A0B2CA7C982C03C1AFBDB3540A918EE937B06CB|cotB]-[gene|61004D2C1EDA3CD84514FD6D468BF594575616F4|ywrJ]
description
[Pubmed|22383849]
regulation
expressed late during sporulation in the mother cell ([wiki|SigE], [protein|search|SigK], [protein|search|GerE], [wiki|SpoIIID]) [Pubmed|26577401,15699190,8755863,15470121]
regulatory mechanism
[protein|0D7F5504590512E1598B94DC1734BF8FF67ED994|gerR]: activation, [Pubmed|20435725], in [regulon|protein:0D7F5504590512E1598B94DC1734BF8FF67ED994|gerR regulon]
[protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: repression, [Pubmed|15470121], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: activation, [Pubmed|26577401], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,8755863,1691789,12480901], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15383836], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
BKE36060 (Δ[gene|D53067098F693669F61E5CCF6C06664580C40C79|cotH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCATAATCCTCCTTACA, downstream forward: _UP4_TGAATGCGTGAAAATGGGTA
BKK36060 (Δ[gene|D53067098F693669F61E5CCF6C06664580C40C79|cotH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCATAATCCTCCTTACA, downstream forward: _UP4_TGAATGCGTGAAAATGGGTA
References
Reviews
Original Publications
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Time of last update: 2024-10-15 11:24:35
Author of last update: Jstuelk