rnhB
168
RNase HII, endoribonuclease, responsible for removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication
locus
BSU_16060
Molecular weight
28.20 kDa
pI
5.52
function
endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules
product
RNase HII
essential
no
ec
3.1.26.4
synonyms
rnhB, rnh
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0164 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,677,451 → 1,678,218
Phenotypes of a mutant
increased mutation rate [Pubmed|23882084], strand- and sequence-context–dependent GC → AT transitions [pubmed|29078353]
a ''[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB] [gene|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC]'' double mutant grows poorly [Pubmed|23882084]
a ''[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB] [gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]'' mutant is not viable [Pubmed|23882084]
The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage to 5'-phosphomonoester (according to UniProt)
incises DNA-RNA duplexes at aingle rNMPs [pubmed|29078353]
removal of single rNMPs incorporated into DNA by DNA polymerase during [wiki|DNA replication] [Pubmed|23882084]
Protein family
[wiki|RNase] HII family (with [protein|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC], according to UniProt)
[wiki|Cofactors]
Mg2+, Mn2+ [pubmed|29084857]
Structure
[PDB|2ETJ] (from Thermotoga maritima, corresponding to aa 69 ... 250, 47% identity)
[AF|O31744]
[wiki|Localization]
recruited to the replication forks [pubmed|39195267]
Expression and Regulation
Operons
genes
[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB]-[gene|0D4A4A1B2FD9D0698D0A65010A8ABC84DA7386A2|ylqG]-[gene|3F271D9FFE4F30D845319CD71662695AA592EC8F|ylqH]
description
[Pubmed|26577401]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|26577401], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
genes
[gene|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA]-[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB]-[gene|0D4A4A1B2FD9D0698D0A65010A8ABC84DA7386A2|ylqG]-[gene|3F271D9FFE4F30D845319CD71662695AA592EC8F|ylqH]
description
[Pubmed|22383849]
Biological materials
Mutant
BP423 (''ΔrnhB::aphA3'') available in [wiki|Fabian Commichau]'s lab
BKE16060 (Δ[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTCTCTTCTCCCTTAC, downstream forward: _UP4_TAAATCACCATGGACAAGGA
BKK16060 (Δ[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTCTCTTCTCCCTTAC, downstream forward: _UP4_TAAATCACCATGGACAAGGA
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], based on [wiki|pGP380], expression from plasmid: pBP500 (E. coli amp & B. subtilis E/L) , available in [wiki|Fabian Commichau]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Fabian Commichau]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-31 04:34:14
Author of last update: Jstuelk