rnhB

rnhB
168

RNase HII, endoribonuclease, responsible for removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication

locus
BSU_16060
Molecular weight
28.20 kDa
pI
5.52
Protein length
Gene length
function
endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules
product
RNase HII
essential
no
ec
3.1.26.4
synonyms
rnhB, rnh

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0164 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,677,451 → 1,678,218
Phenotypes of a mutant
increased mutation rate [Pubmed|23882084], strand- and sequence-context–dependent GC → AT transitions [pubmed|29078353]
a ''[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB] [gene|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC]'' double mutant grows poorly [Pubmed|23882084]
a ''[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB] [gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]'' mutant is not viable [Pubmed|23882084]
The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage to 5'-phosphomonoester (according to UniProt)
incises DNA-RNA duplexes at aingle rNMPs [pubmed|29078353]
removal of single rNMPs incorporated into DNA by DNA polymerase during [wiki|DNA replication]  [Pubmed|23882084]
Protein family
[wiki|RNase] HII family (with [protein|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC], according to UniProt)
[wiki|Cofactors]
Mg2+, Mn2+ [pubmed|29084857]
Structure
[PDB|2ETJ] (from Thermotoga maritima, corresponding to aa 69 ... 250, 47% identity)
[AF|O31744]
[wiki|Localization]
recruited to the replication forks [pubmed|39195267]
Expression and Regulation
Operons
genes
[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB]-[gene|0D4A4A1B2FD9D0698D0A65010A8ABC84DA7386A2|ylqG]-[gene|3F271D9FFE4F30D845319CD71662695AA592EC8F|ylqH]
description
[Pubmed|26577401]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|26577401], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
Open in new tab

[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB]→[gene|3F271D9FFE4F30D845319CD71662695AA592EC8F|ylqH]

2024-10-11 17:54:46

ghost

89

98f7ebb4fcf1623da9e58287f3624156459fba8c

1CC90F1BF46AECEE0BD7591672B817F5E16299E6

genes
[gene|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA]-[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB]-[gene|0D4A4A1B2FD9D0698D0A65010A8ABC84DA7386A2|ylqG]-[gene|3F271D9FFE4F30D845319CD71662695AA592EC8F|ylqH]
description
[Pubmed|22383849]
Open in new tab

[gene|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA]→[gene|3F271D9FFE4F30D845319CD71662695AA592EC8F|ylqH]

2024-10-06 06:56:07

Jstuelk

91

eeb1b6a4de31a5049ef87638911db24d52f44a7c

3D86AE4ED34D50165869C1951375B5B0E636471C

Biological materials
Mutant
BP423 (''ΔrnhB::aphA3'') available in [wiki|Fabian Commichau]'s lab
BKE16060 (Δ[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTCTCTTCTCCCTTAC,  downstream forward: _UP4_TAAATCACCATGGACAAGGA
BKK16060 (Δ[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTCTCTTCTCCCTTAC,  downstream forward: _UP4_TAAATCACCATGGACAAGGA
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], based on [wiki|pGP380], expression from plasmid: pBP500 (E. coli amp & B. subtilis E/L) , available in [wiki|Fabian Commichau]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Fabian Commichau]'s lab
References
Reviews
19228197,24464998,31464530
Original Publications
9888800,10094689,12417313,17905985,23882084,26577401,29078353,29084857,39195267

D5275ECDC2A70FBAC2CA5980F04B272C3328FC90

Page visits: 3844

Time of last update: 2024-10-11 17:06:08

Author of last update: Jstuelk