pheA
168
prephenate dehydratase
locus
BSU_27900
Molecular weight
31.75 kDa
pI
5.89
function
biosynthesis of phenylalanine
product
prephenate dehydratase
essential
no
ec
4.2.1.51
synonyms
pheA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0077 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,851,283 → 2,852,140
The protein
Catalyzed reaction/ biological activity
H+ + prephenate --> 3-phenylpyruvate + CO2 + H2O (according to UniProt)
[wiki|Domains]
Prephenate dehydratase domain (aa 2-183) (according to UniProt)
[wiki|ACT domain] (aa 204-281) (according to UniProt)
Structure
[PDB|4LUB] (from ''Streptococcus mutans'', 42% identity)
[AF|P21203]
Effectors of protein activity
subject to feedback inhibtion by phenylalanine [Pubmed|4956345]
Expression and Regulation
Operons
genes
[gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]-[gene|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg]-[gene|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]-[gene|D4CCF6727AAC048792E59717E588C36EB617249D|pheA]
description
[Pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2537815], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
expression is repressed by binding of [wiki|RpoE] to the A-rich sequence in the -35 region of the promoter [Pubmed|27679485]
Biological materials
Mutant
1A617 ( ''pheA''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A617&Search=1A617 BGSC]
BKE27900 (Δ[gene|D4CCF6727AAC048792E59717E588C36EB617249D|pheA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GACTTTCATGACGATTTTCT, downstream forward: _UP4_TAAAAAAAGCCCACTAGAGG
BKK27900 (Δ[gene|D4CCF6727AAC048792E59717E588C36EB617249D|pheA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GACTTTCATGACGATTTTCT, downstream forward: _UP4_TAAAAAAAGCCCACTAGAGG
References
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Time of last update: 2025-04-02 23:29:01
Author of last update: Melvin.boenninger