qcrA
168
Rieske factor, menaquinol:cytochrome c oxidoreductase (iron-sulfur subunit), component of the cytochrome bc1 complex
locus
BSU_22560
Molecular weight
18.59 kDa
pI
6.08
function
respiration
product
menaquinol:cytochrome c oxidoreductase (iron-sulfur subunit)
essential
no
synonyms
qcrA, bfcA, petC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0723 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,364,589 → 2,365,092
Phenotypes of a mutant
severe growth defect [pubmed|31420537]
The protein
[wiki|Domains]
Rieske domain (aa 59-158) (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
Structure
[PDB|2ZT9] (from Nostoc sp., 31% identity) [pubmed|19189962]
[AF|P46911]
[wiki|Localization]
cell membrane [Pubmed|23880299]
delivered to the membrane by the [protein|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY]-[protein|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY] [wiki|protein secretion] system, this depends on prior dusulfide bond formation and co-factor insertion [Pubmed|24652282,23256564]
Expression and Regulation
Operons
genes
[gene|D4AF96C936093BA44E46FF92A3EDE437495B0923|qcrA]-[gene|1DC5C6DAF0AB1EB59BC9361DC1161E75F99AFE33|qcrB]-[gene|F2973C512AFB4B9F8FE0CCAF09646E60F886C013|qcrC]
description
[Pubmed|7592464]
regulation
induced by oxygen limitation ([protein|search|ResD]) [Pubmed|8631715]
regulatory mechanism
[protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]: activation, [Pubmed|8631715], in [regulon|protein:1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7592464], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
highly expressed at high iron concentrations [pubmed|31420537]
Biological materials
Mutant
BKE22560 (Δ[gene|D4AF96C936093BA44E46FF92A3EDE437495B0923|qcrA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTTCTCCCCCCTCTA, downstream forward: _UP4_GCAAAGCCTAAGGGGGAAGG
BKK22560 (Δ[gene|D4AF96C936093BA44E46FF92A3EDE437495B0923|qcrA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTTCTCCCCCCTCTA, downstream forward: _UP4_GCAAAGCCTAAGGGGGAAGG
References
Reviews
Original Publications
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Time of last update: 2024-12-20 07:41:32
Author of last update: Jstuelk