ganS
168
galactotriose [wiki|ABC transporter], binding protein
locus
BSU_34160
Molecular weight
45.38 kDa
pI
5.44
function
uptake of galactotriose
product
galactotriose [wiki|ABC transporter], binding protein
essential
no
synonyms
cycB, yvfK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2182 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,507,253 → 3,508,518
Phenotypes of a mutant
a ''[gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]-[gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]-[gene|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|ganQ]-[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]-[gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]'' mutant is impaired in the utilization of galactan [Pubmed|22893383]
The protein
Protein family
[wiki|Bacterial solute-binding protein 1 family] (according to UniProt)
Structure
[PDB|2ZYK] (from Thermoactinomyces vulgaris, 38% identity) [pubmed|17371546]
[AF|O07009]
Paralogous protein(s)
[protein|5771E1976D986638B13ADDE97EDE32709E355923|mdxE]
[wiki|Localization]
cell membrane (via [protein|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]-[protein|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|ganQ]) [Pubmed|10092453]
Expression and Regulation
Operons
genes
[gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]-[gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]-[gene|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|ganQ]-[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]-[gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]
description
[Pubmed|9287030]
regulation
induced by galactan (binding of galactobiose to [protein|1F787F70C87DEB221709579122D63C2322A84E56|ganR]) [Pubmed|28617843,27501980]
repressed by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|28617843,27501980]
regulatory mechanism
[protein|1F787F70C87DEB221709579122D63C2322A84E56|ganR]: repression, [Pubmed|9287030], in [regulon|protein:1F787F70C87DEB221709579122D63C2322A84E56|ganR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [pubmed|28617843], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|27501980], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A484 (yvfK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/484 NBRP B. subtilis, Japan]
BKE34160 (Δ[gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATTGTCACCCTTTCG, downstream forward: _UP4_TAAAGAGAAAGCTAGATTTG
BKK34160 (Δ[gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATTGTCACCCTTTCG, downstream forward: _UP4_TAAAGAGAAAGCTAGATTTG
References
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Time of last update: 2024-12-21 15:33:21
Author of last update: Jstuelk