polX
168
DNA polymerase X, involved in DNA repair, generates adaptive mutations by error-prone processing of A/P sites
locus
BSU_28590
Molecular weight
63.95 kDa
pI
5.31
function
DNA repair
product
DNA polymerase X
essential
no
ec
2.7.7.7
synonyms
polX, yshC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1796 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,923,314 → 2,925,026
The protein
Catalyzed reaction/ biological activity
template-dependent DNA polymerase, fills single nucleotide gaps [Pubmed|18938175]
has intrinsic 3'-5' exonuclease activity for resecting unannealed 3'-termini in gapped DNA substrates [Pubmed|18776221]
has intrinsic apurinic/apyrimidinic (AP) endonuclease activity [Pubmed|20974932]
2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
Protein family
N-terminal part: DNA polymerase type-X family (single member, according to UniProt)
C-terminal part: PHP family (single member, according to UniProt)
[wiki|Domains]
C-terminal PHP domain with an active site formed by nine histidines and aspartates that catalyzes 3'-5' exonuclease, AP-endonuclease, 3'-phosphodiesterase and 3'-phosphatase activities [pubmed|31289311]
Structure
[PDB|3AU2] (from Thermus thermophilus, complex with dGTP, 37% identity) [pubmed|22306405]
[AF|P94544]
Expression and Regulation
Operons
genes
[gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]-[gene|93726C423313C653C4F71CB107FA12F686959E41|yshB]-[gene|D3954F2BCF6CE66505E4F48FCB4A61EE8D24F6D3|polX]-[gene|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU]-[gene|18CEF3F1EE2E95A009699DE370CBB44F025B7B40|yshE]
description
[Pubmed|22383849]
regulation
constitutively expressed [Pubmed|23701187]
Biological materials
Mutant
GP3560 (Δ([gene|D3954F2BCF6CE66505E4F48FCB4A61EE8D24F6D3|polX]-[gene|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU])::kan), available in [wiki|Jörg Stülke]'s lab
MGNA-B003 (yshC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1002 NBRP B. subtilis, Japan]
BKE28590 (Δ[gene|D3954F2BCF6CE66505E4F48FCB4A61EE8D24F6D3|polX]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCATAACCCCCAGACG, downstream forward: _UP4_TAAGTAAGGAGGCTCACACA
BKK28590 (Δ[gene|D3954F2BCF6CE66505E4F48FCB4A61EE8D24F6D3|polX]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCATAACCCCCAGACG, downstream forward: _UP4_TAAGTAAGGAGGCTCACACA
labs
[wiki|Margarita Salas], Madrid, Spain [http://www.cbm.uam.es/mkfactory.esdomain/webs/CBMSO/plt_LineasInvestigacion.aspx?IdObjeto=28 link]
References
Reviews
Original Publications
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Time of last update: 2025-04-17 09:38:12
Author of last update: MBenda