gtaB
168
major vegetative UTP-glucose-1-phosphate uridylyltransferase, general stress protein
locus
BSU_35670
Molecular weight
32.92 kDa
pI
4.91
function
biosynthesis of teichoic acid
product
UTP-glucose-1-phosphate uridylyltransferase
essential
no
ec
2.7.7.9
synonyms
gtaB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1210 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,665,629 → 3,666,507
Phenotypes of a mutant
cells are bent and distended due to the lack of glucolipids [Pubmed|27684739]
the inactivation of ''[gene|D368988F85D8436DD424B0B4A1591BDF092A8EA7|gtaB]'' suppresses the poor and filametous growth of the ''[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]'' double mutant [Pubmed|24097947]
inactivation of ''[gene|D368988F85D8436DD424B0B4A1591BDF092A8EA7|gtaB]'' reduces sporulation efficiency to 0.1% that of wild type cells; smaller, wider mother cells with small forespores [Pubmed|26735940]
resistant to phage SPO1 [pubmed|30811056]
defective in [category|SW.4.1.4|Swarming] motility (periodic cessation and reinitiation of [category|SW.4.1.4|Swarming] motility, terracing colonies) [pubmed|35638827]
The protein
Catalyzed reaction/ biological activity
produces UDP-glucose from UTP and glucose-1-phosphate [pubmed|33556370]
Protein family
UDPGP type 2 family (with [protein|69C5D9EF7ED43BCAB7A9D6148EDD323CE72F8E3D|yngB] and [protein|FC455B727F66632D94D93E103B30748944ED04A0|ytdA], according to UniProt)
Structure
[PDB|7B1R] ([protein|69C5D9EF7ED43BCAB7A9D6148EDD323CE72F8E3D|yngB], 58% identity) [pubmed|33556370]
[PDB|2E3D] (from ''E. coli'', 44% identity, 61% similarity) [Pubmed|17322528]
[AF|Q05852]
Modification
phosphorylated on Arg-76 [Pubmed|22517742]
phosphorylated on ser/ thr/ tyr [Pubmed|16493705]
Paralogous protein(s)
[protein|FC455B727F66632D94D93E103B30748944ED04A0|ytdA], [protein|69C5D9EF7ED43BCAB7A9D6148EDD323CE72F8E3D|yngB]
Expression and Regulation
Operons
genes
[gene|D368988F85D8436DD424B0B4A1591BDF092A8EA7|gtaB]
description
[Pubmed|8320212]
regulation
induced by stress ([protein|search|SigB]) [Pubmed|8320212,11544224]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8320212], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|8320212,11544224], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
BKE35670 (Δ[gene|D368988F85D8436DD424B0B4A1591BDF092A8EA7|gtaB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTAAAAGGCACCTTCCT, downstream forward: _UP4_TAAACAAAAAGGCTATTGGA
BKK35670 (Δ[gene|D368988F85D8436DD424B0B4A1591BDF092A8EA7|gtaB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTAAAAGGCACCTTCCT, downstream forward: _UP4_TAAACAAAAAGGCTATTGGA
References
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Time of last update: 2024-12-21 16:40:43
Author of last update: Jstuelk