yfjQ
168
minor magnesium transporter
locus
BSU_08000
Molecular weight
37.69 kDa
pI
5.42
function
magnesium uptake
product
minor magnesium transporter
essential
no
synonyms
yfjQ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0598 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
871,347 → 872,306
Phenotypes of a mutant
a [gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE] [gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ] mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medium in the presence of citrate (due to the activity of [protein|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]) [Pubmed|24415722]
The protein
Protein family
CorA metal ion transporter (MIT) (TC 1.A.35) family (together with [protein|F0CACEFB53F4BC38DF77A38C4D78BA3DBE96E21A|corA]) (according to UniProt)
Structure
[PDB|4I0U] (from Thermotoga maritima, 37% identity) [pubmed|23425532]
[AF|O31543]
Expression and Regulation
Operons
genes
[gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-C274 (yfjQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2272 NBRP B. subtilis, Japan]
BKE08000 (Δ[gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAACCCTCCACCTG, downstream forward: _UP4_TGAGGACAGCGAGCAGCTGT
BKK08000 (Δ[gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAACCCTCCACCTG, downstream forward: _UP4_TGAGGACAGCGAGCAGCTGT
GP2853 (''[gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ]''::''tet''), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-12-19 16:49:31
Author of last update: Jstuelk