conE
168
ATPase, required for conjugation of ICEBs1, part of the type IV secretion system for DNA transfer
locus
BSU_04940
Molecular weight
96.06 kDa
pI
5.51
function
conjugative transfer of ICEBs1
product
ATPase, part of the type IV secretion system for DNA transfer
essential
no
ec
null
synonyms
conE, yddE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3451 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
538,836 → 541,229
Phenotypes of a mutant
Defective for conjugal transfer of ICE''Bs1
The protein
Catalyzed reaction/ biological activity
required for conjugative transfer of ICEBs1, putative ATPase [Pubmed|19734305]
Protein family
HerA/FtsK Superfamily of ATPases including the well-studied proteins FtsK, SpoIIIE, VirB4, and VirD4
Structure
[AF|P96642]
[wiki|Localization]
peripheral membrane protein (via [protein|A0AFD543582D2FE8B1139CF69B7B1F7FF0F80265|conB]), located at the cell poles [Pubmed|26013486,19734305]
Expression and Regulation
Operons
genes
[gene|EE2A4068CBD7A2F852E582E2EDA4148381F1CD61|xis]-[gene|2227923C603B290AB276238F789D71584FB07DF3|ydzL]-[gene|34451538F10B4ABCFC8A236C8C905A20D989EBCF|devI]-[gene|C042792D9A643DCFF4A851EFECA3CF2C31E1EDAF|ydcP]-[gene|4BCAA5939CB7F0DF23495BC92C9EF0A0149296A0|ydcQ]-[gene|8E179976C35FE09F92A191D00E497ED4BF28F571|nicK]-[gene|886B2132717BA4C72433ABE351C5791C9DE1432A|S172]-[gene|1699CFBF5400CAF43C6DAE8ADCCE5A360B31F2E9|ydcS]-[gene|9D4918852A1856B1CDD72D49AEA75E51A8602BB6|ydcT]-[gene|62CEABA069CA2970F22B7E0C369D540A2A027A8D|yddA]-[gene|A0AFD543582D2FE8B1139CF69B7B1F7FF0F80265|conB]-[gene|F273A8817E66B44D58FC2BCB6576A30D8616B750|conC]-[gene|F1F898D7A9FDF78D2E5E8F3BF605E0F735D0542A|conD]-[gene|D20F724D8C3E8C758A9FADF133CD43F066BD8D48|conE]-[gene|B3A07E2A61151C33B7C65D1BC58B97BFDB76852D|yddF]-[gene|3356A43B83E2D4D66098F17A751F0912C1553398|conG]-[gene|8D9D81E05BAA8697F002BB1B8DBC5ABBA557353F|cwlT]-[gene|2E4FF80C857B3F40BA835814EC40D6562721AEFE|yddI]-[gene|2FB1C9435AE924F8E450AFA81F800196A871F2D0|yddJ]
description
[pubmed|22383849]
regulatory mechanism
[protein|DD1C8F3A4809785BD6A6047D39B42AB2C605E161|immR]: repression, [Pubmed|17511812], in [regulon|protein:DD1C8F3A4809785BD6A6047D39B42AB2C605E161|immR regulon]
Biological materials
Mutant
BKE04940 (Δ[gene|D20F724D8C3E8C758A9FADF133CD43F066BD8D48|conE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTTTTATACGATTCAACTT, downstream forward: _UP4_TTGTAAAAAGGGGTTGAATC
BKK04940 (Δ[gene|D20F724D8C3E8C758A9FADF133CD43F066BD8D48|conE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTTTTATACGATTCAACTT, downstream forward: _UP4_TTGTAAAAAGGGGTTGAATC
labs
[wiki|Melanie Barker Berkmen], Suffolk University, Boston, MA [http://openwetware.org/wiki/Berkmen Homepage]
References
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Time of last update: 2024-10-04 06:40:28
Author of last update: Jstuelk