ilvA
168
threonine dehydratase
locus
BSU_21770
Molecular weight
46.49 kDa
pI
5.54
function
biosynthesis of branched-chain amino acids
product
threonine dehydratase
essential
no
ec
4.3.1.19
synonyms
ilvA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1171 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,292,769 → 2,294,037
The protein
Catalyzed reaction/ biological activity
L-threonine --> 2-oxobutanoate + NH4+ (according to UniProt)
Protein family
serine/threonine dehydratase family (with [protein|4E7390A74A8261E8B732E94859E90FE256AD64EE|dsdA], according to UniProt)
[wiki|Domains]
ACT-like domain (aa 339-413) (according to UniProt)
[wiki|Cofactors]
PLP (according to UniProt)
Structure
[PDB|1TDJ] (the enzyme from ''E. coli'', 39% identity) [Pubmed|9562556]
[AF|P37946]
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
Expression and Regulation
Operons
genes
[gene|D0CF32BF81AA1DC7BBBC3E9B667A54F97260DF3F|ilvA]-[gene|DE113BB32600349C472D3417B760D640B65B01AB|ypmP]
description
[Pubmed|15060025]
regulation
repressed during growth in the presence of branched chain amino acids ([protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]) [Pubmed|12618455]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
Biological materials
Mutant
BKE21770 (Δ[gene|D0CF32BF81AA1DC7BBBC3E9B667A54F97260DF3F|ilvA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTACAGATTCCTTTCTT, downstream forward: _UP4_TAATTTTTTTACACAACCGC
BKK21770 (Δ[gene|D0CF32BF81AA1DC7BBBC3E9B667A54F97260DF3F|ilvA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTACAGATTCCTTTCTT, downstream forward: _UP4_TAATTTTTTTACACAACCGC
lacZ fusion
pGP519 (in [wiki|pAC5]), available in [wiki|Stülke] lab
References
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Time of last update: 2025-04-03 21:12:16
Author of last update: Melvin.boenninger