azoR1
168
azoreductase, involved in quinone detoxification
locus
BSU_19230
Molecular weight
22.83 kDa
pI
4.99
function
quinone detoxification
product
azoreductase
essential
no
synonyms
azoR1, yocJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1182 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,096,350 2,096,976
The protein
Catalyzed reaction/ biological activity
anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ --> 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 H+ + 2 NADH (according to UniProt)
Protein family
azoreductase type 1 family (with [protein|0E2B1914A040666BAF042C0BE3F468144E1F1E06|azoR2], according to UniProt)
Structure
[PDB|3P0R] (from B. anthracis, 64% identity, 81% similarity)
[AF|O35022]
Paralogous protein(s)
[protein|0E2B1914A040666BAF042C0BE3F468144E1F1E06|azoR2]
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|CFFFFE96FB7AEEF7190F81332369F0A6834C34A4|azoR1]
description
[pubmed|22383849]
regulation
induced by thiol-reactive compounds (catechol, menaquinone) ([protein|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|yodB]) [pubmed|18208493]
regulatory mechanism
[protein|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|yodB]: repression, [pubmed|18208493], in [regulon|protein:73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|yodB regulon]
Biological materials
Mutant
MGNA-B415 (yocJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1414 NBRP B. subtilis, Japan]
BKE19230 ([gene|CFFFFE96FB7AEEF7190F81332369F0A6834C34A4|azoR1]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTATTCCTCCCGGTA, downstream forward: _UP4_TAATAAAGCCAAGACTCCTA
BKK19230 ([gene|CFFFFE96FB7AEEF7190F81332369F0A6834C34A4|azoR1]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTATTCCTCCCGGTA, downstream forward: _UP4_TAATAAAGCCAAGACTCCTA
References
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Time of last update: 2024-12-19 18:40:29
Author of last update: Melvin.boenninger