ispD
168
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, third step in the MEP pathway of isoprenoid biosynthesis
locus
BSU_00900
Molecular weight
25.70 kDa
pI
5.58
function
MEP pathway of isoprenoid biosynthesis
product
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
essential
yes
ec
2.7.7.60
synonyms
ispD, yacM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1211 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
109,789 → 110,487
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
2-C-methyl-D-erythritol 4-phosphate + CTP + H+ --> 4-CDP-2-C-methyl-D-erythritol + diphosphate (according to UniProt)
Protein family
cytidylyltransferase family (with [protein|8620DA76A1953D7ACDFA7E46ED81EF1A0D97999C|tagD] and [protein|B25AE6979AF12628780C4489FFED966C6B31968A|yfnH], according to UniProt)
IspD/TarI cytidylyltransferase family (single member, according to UniProt)
Structure
[PDB|5DDT] [Pubmed|27821871]
[PDB|5DDV] [Pubmed|27821871]
[AF|Q06755]
Expression and Regulation
Operons
genes
[gene|179FDEA51A060DF32A3EA06A796793DE27B600FF|yacL]-[gene|CF9633F769263C6D16EE778F6BD2ECF1995F512A|ispD]-[gene|1A6793A3A5AC568C67873C8708E9F581F42AB9BA|ispF]
description
[Pubmed|10482513]
regulation
induced by stress ([protein|search|SigB]) [Pubmed|15805528]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|17434969], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
Biological materials
Mutant
MGNA-B881 (yacM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1880 NBRP B. subtilis, Japan]
References
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Time of last update: 2024-12-21 15:07:26
Author of last update: Melvin.boenninger