prmC
168
similar to N5-glutamine methyltransferase that modifies peptide release factors [protein|59E6468FD891F5271F38543515867B0B4625768D|prfA] and [protein|12E06FD3A36D95AE82E3C65788FA98178039592F|prfB]
locus
BSU_37000
Molecular weight
32.23 kDa
pI
4.89
function
peptide release factor methylation
product
N5-glutamine methyltransferase
essential
no
ec
2.1.1.297
synonyms
prmC, hemK, ywkE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2890 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,796,217 → 3,797,083
Phenotypes of a mutant
non-transformable [pubmed|28189581]
defective in swarming motility (reduced rate of colony expansion) [pubmed|35638827]
additional information
overexpression results in activation of [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB] [pubmed|33900014]
The protein
Catalyzed reaction/ biological activity
L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine --> H+ + N5-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
protein N5-glutamine methyltransferase family (single member, according to UniProt)
Structure
[PDB|1NV8] (the protein from ''Thermotoga maritima'') [Pubmed|12741815]
[AF|P45873]
Expression and Regulation
Operons
genes
[gene|59E6468FD891F5271F38543515867B0B4625768D|prfA]-[gene|CF959D90A9B8F83E40E7B81CBFB00B5EC09EC0DD|prmC]-[gene|B56B38A1CCC0A73FFD9F3F4C9942356E6D818D2D|yfkF]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A199 (ywkE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/199 NBRP B. subtilis, Japan]
BKE37000 (Δ[gene|CF959D90A9B8F83E40E7B81CBFB00B5EC09EC0DD|prmC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAGGGCTTCGAATATCGTCT, downstream forward: _UP4_TAAGCAGCTTGCTGGCGAGT
BKK37000 (Δ[gene|CF959D90A9B8F83E40E7B81CBFB00B5EC09EC0DD|prmC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAGGGCTTCGAATATCGTCT, downstream forward: _UP4_TAAGCAGCTTGCTGGCGAGT
References
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Time of last update: 2024-12-21 14:24:49
Author of last update: Jstuelk