sda
168
developmental checkpoint protein, controls the phosphorylation status of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]
locus
BSU_25690
Molecular weight
6.02 kDa
pI
5.61
function
mediates a developmental checkpoint coupling initiation of [category|SW.4.2|Sporulation] (phosphorylation of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A])to the function of replication initiation proteins
product
developmental checkpoint protein
essential
no
synonyms
sda
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5851 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,647,456 → 2,647,614
Phenotypes of a mutant
sporulates at a higher frequency
will sporulate in the presence of replication stress
The protein
Catalyzed reaction/ biological activity
Sda is a checkpoint protein that is upregulated in response to replication stress [Pubmed|11207367]. Sda inhibits the autokinase activity of [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA] (and likely [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|kinB] as well). This in its turn results in reduced levels of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A~P]]]. Thus, replication stressed cells will not initiate sporulation.
Structure
[PDB|3D36] (from ''Geobacillus stearothermophilus'', complex with [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|kinB]) [Pubmed|19101565], [PDB|1PV0]
[AF|Q7WY62]
Expression and Regulation
Operons
genes
[gene|CEFD10FAFA0DBC83CD2B61DAC9339FEE025B1611|sda]
description
[Pubmed|11207367]
regulation
induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]
regulatory mechanism
[protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]: activation, under replication stress [Pubmed|17932079], in [regulon|protein:6740108089F13116F200C15F35C2E7561E990FEB|dnaA regulon]
[protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11207367], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
An [wiki|antisense RNA] is predicted for '[protein|search|sda]' [PubMed|20525796]
Biological materials
Mutant
1A1034 (no resistance), [Pubmed|16796683], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1034&Search=1A1034 BGSC]
BKE25690 (Δ[gene|CEFD10FAFA0DBC83CD2B61DAC9339FEE025B1611|sda]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATGGGAGAGGCACCTCCTT, downstream forward: _UP4_TAATAGGAATTTGTCTATTT
BKK25690 (Δ[gene|CEFD10FAFA0DBC83CD2B61DAC9339FEE025B1611|sda]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATGGGAGAGGCACCTCCTT, downstream forward: _UP4_TAATAGGAATTTGTCTATTT
labs
[wiki|Bill Burkholder] [http://burkholder.stanford.edu/ Homepage]
References
Reviews
Original Publications
Page visits: 3651
Time of last update: 2024-10-05 20:58:46
Author of last update: Jstuelk