safA
168
morphogenetic protein associated with [protein|6698BB092E03BEF48AFD0CBF565410109CD1ABB5|spoVID], major organizer of the inner spore coat
locus
BSU_27840
Molecular weight
43.07 kDa
pI
5.75
function
spore coat formation
product
morphogenetic protein
essential
no
synonyms
safA, yrbA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1388 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,844,675 → 2,845,838
Phenotypes of a mutant
mis-assembly of the inner spore coat [Pubmed|22171814]
The protein
[wiki|Domains]
contains a N-acetylglucosamine-polymer-binding [wiki|LysM domain] at the N-terminus [Pubmed|18430080]
[wiki|LysM domain] (aa 2-47) (according to UniProt)
Structure
[AF|O32062]
[wiki|Localization]
inner spore coat [Pubmed|23202530]
cortex-coat interface [pubmed|29712873]
Expression and Regulation
Operons
genes
[gene|CEBEC9CECCF445C40D793E61A2CD23175631A473|safA]-[gene|6EAB09018D9AF9C488E713C232CD77B63415DCE3|coxA]
description
[Pubmed|10438771]
regulation
expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|10438771]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|10438771], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
MGNA-A011 (yrbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/11 NBRP B. subtilis, Japan]
1S126 ( ''safA''::''erm''), [Pubmed|16751597], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S126&Search=1S126 BGSC]
1S117 ( ''safA''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S117&Search=1S117 BGSC]
BKE27840 (Δ[gene|CEBEC9CECCF445C40D793E61A2CD23175631A473|safA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGTTTTCCCCTCCTATG, downstream forward: _UP4_TGATCGTTCGGAACGATGTA
BKK27840 (Δ[gene|CEBEC9CECCF445C40D793E61A2CD23175631A473|safA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGTTTTCCCCTCCTATG, downstream forward: _UP4_TGATCGTTCGGAACGATGTA
labs
[wiki|Adriano Henriques], Lisbon, Portugal [http://www.itqb.unl.pt/research/biology/microbial-development homepage]
[wiki|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-30 11:52:32
Author of last update: Jstuelk