melA
168
alpha-galactosidase
locus
BSU_30300
Molecular weight
49.10 kDa
pI
5.84
function
utilization of melibiose and raffinose family oligosaccharides (raffinose, stachyose)
product
alpha-galactosidase
essential
no
ec
3.2.1.22
synonyms
melA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1486 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,100,881 → 3,102,179
The protein
Catalyzed reaction/ biological activity
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids (according to UniProt)
Protein family
[wiki|Glycosyl hydrolase 4 family] (according to UniProt)
[wiki|Cofactors]
NAD+ [pubmed|31138628]
Mn2+ [pubmed|31138628]
Structure
[PDB|5C3M] (from Geobacillus stearothermophilus, 28% identity)
[AF|O34645]
Kinetic information
KM for melibiose: 10 mM [pubmed|31138628]
KM for raffinose: 25 mM [pubmed|31138628]
Expression and Regulation
Operons
genes
[gene|CDE9EF9CFB799794D525F12DEC8C4190C6298575|melA]
description
[Pubmed|22383849,9387221]
regulation
repressed by glucose ([protein|search|CcpA]) [Pubmed|22900538]
genes
[gene|C3D7C64AD0E58BF326257E23136466A237138B50|melE]-[gene|05BFEA711D82395A55B505E5A38286BE2F570350|melD]-[gene|4E766839240C8F7D56D87DF9E7B99350A83B5FF7|melC]-[gene|CDE9EF9CFB799794D525F12DEC8C4190C6298575|melA]
description
[Pubmed|22383849]
regulation
repressed by glucose ([protein|search|CcpA]) [Pubmed|22900538]
genes
[gene|58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|melR]-[gene|C3D7C64AD0E58BF326257E23136466A237138B50|melE]-[gene|05BFEA711D82395A55B505E5A38286BE2F570350|melD]-[gene|4E766839240C8F7D56D87DF9E7B99350A83B5FF7|melC]-[gene|CDE9EF9CFB799794D525F12DEC8C4190C6298575|melA]
description
[Pubmed|22383849]
regulation
repressed by glucose ([protein|search|CcpA]) [Pubmed|22900538]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|22900538], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|melR]: repression, [pubmed|31138628], in [regulon|protein:58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|melR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22900538,31138628], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE30300 (Δ[gene|CDE9EF9CFB799794D525F12DEC8C4190C6298575|melA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATATTGCTTCCCCCTT, downstream forward: _UP4_TAAAAACTAGGGGACCGCTC
BKK30300 (Δ[gene|CDE9EF9CFB799794D525F12DEC8C4190C6298575|melA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATATTGCTTCCCCCTT, downstream forward: _UP4_TAAAAACTAGGGGACCGCTC
References
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Time of last update: 2024-12-03 22:26:01
Author of last update: Melvin.boenninger