ndhF
168
subunit of a Na+:bicarbonate transporter [protein|CCDDC414E266D86B9027EA67EA565D87357023B3|MpsA]-[protein|E04C67FB0EA3FFEC8FB68440F4661505C3DFDA29|MpsB]
locus
BSU_01830
Molecular weight
55.30 kDa
pI
10.15
function
uptake of bicarbonate
product
subunit of a Na+:bicarbonate transporter
essential
no
synonyms
ndhF, ybxE, mpsA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1009 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
205,409 → 206,926
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
severe growth defect [pubmed|31420537]
inactivation of [gene|CCDDC414E266D86B9027EA67EA565D87357023B3|ndhF] facilitates the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate [pubmed|33481774]
The protein
Catalyzed reaction/ biological activity
uptake of bicarbonate (with [protein|E04C67FB0EA3FFEC8FB68440F4661505C3DFDA29|MpsB]) [pubmed|34287032]
Protein family
complex I subunit 5 family (single member, according to UniProt)
[wiki|Cofactors]
FMN or FAD [Pubmed|21635694]
Structure
[PDB|4HE8] (from Thermus thermophilus, corresponds to aa 70 ... 352, 28.% identity) [pubmed|23417064]
[AF|P39755]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|CCDDC414E266D86B9027EA67EA565D87357023B3|ndhF]-[gene|E04C67FB0EA3FFEC8FB68440F4661505C3DFDA29|ybcC]-[gene|5D96DF2F32050BBCEC3811843398FE84DE88F6DF|ybcF]-[gene|EBE0360D89E50EEBB84CC853C6E4B4053DBF8925|ybcH]-[gene|6E063903E9E3B770CAACA9DF98DFA540FAE6FD3D|ybcI]
description
[Pubmed|22383849]
additional information
highly expressed at high iron concentrations [pubmed|31420537]
Biological materials
Mutant
BKE01830 (Δ[gene|CCDDC414E266D86B9027EA67EA565D87357023B3|ndhF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTAAATTCTCCCTTTT, downstream forward: _UP4_ATTTCATAAGGAGCTAATCT
BKK01830 (Δ[gene|CCDDC414E266D86B9027EA67EA565D87357023B3|ndhF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTAAATTCTCCCTTTT, downstream forward: _UP4_ATTTCATAAGGAGCTAATCT
References
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Time of last update: 2024-12-30 11:09:29
Author of last update: Jstuelk