yfnI
168
polyglycerolphosphate lipoteichoic acid synthase, general stress protein, major secreted protein, required for survival at low temperature (4°C)
locus
BSU_07260
Molecular weight
73.14 kDa
pI
5.95
function
biosynthesis of lipoteichoic acid
product
minor lipoteichoic acid synthetase
essential
no
synonyms
yfnI, yetP, ltaSA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1368 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
796,314 → 798,233
Phenotypes of a mutant
induction of [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM] and [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX] activities [Pubmed|23103977]
more sensitive to nisin [Pubmed|23980836]
increased conjugation of ICEBs1 [Pubmed|25069588]
suppression of the growth and morphology defects of a [gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR] mutant [pubmed|30478337]
inactivation suppresses the essentiality of [gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl] [pubmed|39185233]
The protein
Protein family
[wiki|LTA synthase family] (according to UniProt)
Structure
[PDB|2W5Q] ([protein|418900B6DBDF3EF300C930B0C75E31B939F3CE00|ltaS] from S. aureus, corresponds to aa 215 ... 632 of [protein|CCD06F344E3C87E77C3D13883CE2B927F372A84E|yfnI]) [pubmed|19168632]
[AF|Q797B3]
Modification
phosphorylation on (Thr-311 OR Ser-312) [Pubmed|17218307]
can be phosphorylated by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC] on Thr-297 in vitro [pubmed|30478337]
Paralogous protein(s)
[protein|2B33F2F9C2FB0767B72A29D060037393C88ACEE5|yqgS], [protein|418900B6DBDF3EF300C930B0C75E31B939F3CE00|ltaS], [protein|880C655DC7149F6EDC21BD2CF8F9F2C9FBEB2CDB|yvgJ]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|CCD06F344E3C87E77C3D13883CE2B927F372A84E|yfnI]
description
[Pubmed|22383849]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|17434969], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
additional information
term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''yfnI'' [Pubmed|27120414]
Biological materials
Mutant
MGNA-C226 (yfnI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2224 NBRP B. subtilis, Japan]
SM-NB (''yfnI-spc''), available in [wiki|Anne Galinier]'s and [wiki|Boris Görke]'s labs
GP1390 ''yfnI::spc'', available in [wiki|Jörg Stülke]'s lab
GP1397 ''yfnI::ermC'', available in [wiki|Jörg Stülke]'s lab
BKE07260 (Δ[gene|CCD06F344E3C87E77C3D13883CE2B927F372A84E|yfnI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCAACCTCTACTTTCT, downstream forward: _UP4_TAAGATGAAAAAGAGCCTTG
BKK07260 (Δ[gene|CCD06F344E3C87E77C3D13883CE2B927F372A84E|yfnI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCAACCTCTACTTTCT, downstream forward: _UP4_TAAGATGAAAAAGAGCCTTG
References
Reviews
Original Publications
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Time of last update: 2024-12-22 02:54:23
Author of last update: Jstuelk