yfnI

yfnI
168

polyglycerolphosphate lipoteichoic acid synthase, general stress protein, major secreted protein, required for survival at low temperature (4°C)

locus
BSU_07260
Molecular weight
73.14 kDa
pI
5.95
Protein length
Gene length
function
biosynthesis of lipoteichoic acid
product
minor lipoteichoic acid synthetase
essential
no
synonyms
yfnI, yetP, ltaSA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1368 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
796,314 → 798,233
Phenotypes of a mutant
induction of [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM] and [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX] activities [Pubmed|23103977]
more sensitive to nisin [Pubmed|23980836]
increased conjugation of ICEBs1 [Pubmed|25069588]
suppression of the growth and morphology defects of a [gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR] mutant [pubmed|30478337]
inactivation suppresses the essentiality of [gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl] [pubmed|39185233]
The protein
Protein family
[wiki|LTA synthase family] (according to UniProt)
Structure
[PDB|2W5Q] ([protein|418900B6DBDF3EF300C930B0C75E31B939F3CE00|ltaS] from S. aureus, corresponds to aa 215 ... 632 of [protein|CCD06F344E3C87E77C3D13883CE2B927F372A84E|yfnI]) [pubmed|19168632]
[AF|Q797B3]
Modification
phosphorylation on (Thr-311 OR Ser-312) [Pubmed|17218307]
can be phosphorylated by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC] on Thr-297 in vitro [pubmed|30478337]
Paralogous protein(s)
[protein|2B33F2F9C2FB0767B72A29D060037393C88ACEE5|yqgS], [protein|418900B6DBDF3EF300C930B0C75E31B939F3CE00|ltaS], [protein|880C655DC7149F6EDC21BD2CF8F9F2C9FBEB2CDB|yvgJ]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|CCD06F344E3C87E77C3D13883CE2B927F372A84E|yfnI]
description
[Pubmed|22383849]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|17434969], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
additional information
term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''yfnI'' [Pubmed|27120414]
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[gene|CCD06F344E3C87E77C3D13883CE2B927F372A84E|yfnI]

2024-12-18 08:51:08

Jstuelk

81

079680e7f1419dc08ad5c0a6f840ffded294561a

7B73A1854210BCB5725A36CADBFE55CE92AEB78A

Biological materials
Mutant
MGNA-C226 (yfnI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2224 NBRP B. subtilis, Japan]
SM-NB (''yfnI-spc''), available in [wiki|Anne Galinier]'s and [wiki|Boris Görke]'s labs
GP1390 ''yfnI::spc'', available in [wiki|Jörg Stülke]'s lab
GP1397 ''yfnI::ermC'', available in [wiki|Jörg Stülke]'s lab
BKE07260 (Δ[gene|CCD06F344E3C87E77C3D13883CE2B927F372A84E|yfnI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGCAACCTCTACTTTCT,  downstream forward: _UP4_TAAGATGAAAAAGAGCCTTG
BKK07260 (Δ[gene|CCD06F344E3C87E77C3D13883CE2B927F372A84E|yfnI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGCAACCTCTACTTTCT,  downstream forward: _UP4_TAAGATGAAAAAGAGCCTTG
References
Reviews
21388439,21255102
Original Publications
17434969,18957862,17218307,19229300,21255105,23103977,23980836,25069588,27120414,19168632,30478337,34910349,39185233

CCD06F344E3C87E77C3D13883CE2B927F372A84E

Page visits: 4627

Time of last update: 2024-12-22 02:54:23

Author of last update: Jstuelk