gidB
168
7-methylguanosine methyltransferase specific for the 16S rRNA, glucose-inhibited division protein
locus
BSU_41000
Molecular weight
26.80 kDa
pI
6.6
function
rRNA modification
product
7-methylguanosine methyltransferase specific for the 16S rRNA
essential
no
synonyms
gidB, rsmG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0357 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,208,870 → 4,209,589
Phenotypes of a mutant
low-level resistance to streptomycin, increased level of streptomycin-resistant rpsL mutants (200 times higher than in the wild type) [Pubmed|17573471]
The protein
Catalyzed reaction/ biological activity
methylates the N7 position of nucleotide G535 in 16S rRNA [Pubmed|17573471]
Protein family
[wiki|Methyltransferase superfamily] (according to UniProt)
Structure
[PDB|1XDZ]
[AF|P25813]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF]-[gene|7809B4F5B9172EBB6C91BDE7B673FB08E82763FF|gidA]-[gene|CCB7EE1D049585979303148FBDE0D42549E648DE|gidB]-[gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc]
description
[Pubmed|11807071]
regulation
constitutively expressed [Pubmed|23701187]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|11948146], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
[protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]: repression, [Pubmed|26020636], in [regulon|protein:6740108089F13116F200C15F35C2E7561E990FEB|dnaA regulon]
Biological materials
Mutant
MGNA-B875 (gidB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1874 NBRP B. subtilis, Japan]
BKE41000 (Δ[gene|CCB7EE1D049585979303148FBDE0D42549E648DE|gidB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE41000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAATATTCATGCCGTCAT, downstream forward: _UP4_TAATTTAGACAGCCTGATAT
BKK41000 (Δ[gene|CCB7EE1D049585979303148FBDE0D42549E648DE|gidB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK41000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAATATTCATGCCGTCAT, downstream forward: _UP4_TAATTTAGACAGCCTGATAT
References
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Time of last update: 2024-10-02 21:21:59
Author of last update: Jstuelk