yqgN
168
5,10-methenyltetrahydrofolate synthetase, 5-formyltetrahydrofolate cyclo-ligase paralog implicated in thiamin metabolism
locus
BSU_24890
Molecular weight
21.27 kDa
pI
5.79
function
detoxification of 5-formyl-THF
product
5,10-methenyltetrahydrofolate synthetase
essential
no
ec
null
synonyms
yqgN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0212 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,573,760 → 2,574,323
The protein
Catalyzed reaction/ biological activity
converts toxic 5-formyl-THF (side product of [protein|BAE8062DA8538C9C83FFBA9684BECE9613EEFD2F|glyA]) back to 5,10-methenyl-THF [pubmed|21538139]
Protein family
5-formyltetrahydrofolate cyclo-ligase family (single member, according to UniProt)
Structure
[PDB|1YDM]
[AF|P54491]
Biological materials
Mutant
MGNA-C460 (yqgN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2458 NBRP B. subtilis, Japan]
BKE24890 (Δ[gene|CB9728D2A23C8F03104FF781D514CB3E6169835E|yqgN]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTCATCCCCCTCGGA, downstream forward: _UP4_TAACTTTCCTGTCCCTTATT
BKK24890 (Δ[gene|CB9728D2A23C8F03104FF781D514CB3E6169835E|yqgN]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTCATCCCCCTCGGA, downstream forward: _UP4_TAACTTTCCTGTCCCTTATT
Original Publications
Page visits: 1249
Time of last update: 2025-04-17 03:58:27
Author of last update: Jstuelk